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Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against Porphyromonas gingivalis
- Source :
- Insects, Volume 12, Issue 5, Insects, Vol 12, Iss 466, p 466 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species Parnassius bremeri, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the P. bremeri larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against Porphyromonas gingivalis, a primary causative organism of periodontitis.
- Subjects :
- antimicrobial peptide
Science
media_common.quotation_subject
Antimicrobial peptides
Parnassius bremeri
Insect
Porphyromonas gingivalis
Microbiology
03 medical and health sciences
Antibiotic resistance
030304 developmental biology
media_common
0303 health sciences
biology
030306 microbiology
Host (biology)
fungi
endangered species
Antimicrobial
biology.organism_classification
Insect Science
transcriptome
Bacteria
Subjects
Details
- ISSN :
- 20754450
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Insects
- Accession number :
- edsair.doi.dedup.....0d66bf24e51ce2370f7c7317f1b7f67d