MOESM1 of Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR

Additional file 1: Figure S1. Principle of BNA-clamp PCR. Forward and reverse primers amplify the targeted mutation. A BNA probe binds to the wild-type allele but not to the mutated allele. The BNA probe selectively inhibits PCR amplification of the wild-type allele. F, fluorescence; Q, quencher.