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A PLCδ1-binding protein, p122RhoGAP, is localized in focal adhesions
- Source :
- Biochemical Society Transactions. 32:1107-1109
- Publication Year :
- 2004
- Publisher :
- Portland Press Ltd., 2004.
-
Abstract
- We have investigated the cellular distribution of p122RhoGAP, a GTPase-activating protein of Rho small GTPase and an activator of phospholipase C-δ1. Immunofluorescence studies demonstrated that endogenous p122 is localized at the tips of actin stress fibres and co-localizes with vinculin in normal rat kidney cells. In immunoprecipitation studies, p122 co-precipitated with vinculin, indicating that p122 is localized at the sites of focal adhesion. We have also shown that the N-terminal half of p122 is responsible for this localization. It is conceivable, therefore, that p122 is involved in the reorganization of the actin cytoskeleton and focal adhesions that regulate cell–substratum adhesion and cell migration.
- Subjects :
- Binding Sites
biology
Immunoprecipitation
GTPase-Activating Proteins
PTK2
Cell migration
macromolecular substances
Vinculin
Kidney
Actin cytoskeleton
Models, Biological
Biochemistry
Rats
Cell biology
Isoenzymes
Focal adhesion
Type C Phospholipases
Cell Adhesion
biology.protein
Animals
Phospholipase C delta
Paxillin
Actin
Protein Binding
Subjects
Details
- ISSN :
- 14708752 and 03005127
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Biochemical Society Transactions
- Accession number :
- edsair.doi.dedup.....0d600434b033b17e8aefa137f103163e
- Full Text :
- https://doi.org/10.1042/bst0321107