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A PLCδ1-binding protein, p122RhoGAP, is localized in focal adhesions

Authors :
Hajime Hirata
Hitoshi Yagisawa
Katsuhisa Kawai
Yui Iwamae
Hideaki Kamata
Masaki Yamaga
Minoru Kiyota
Yoshimi Homma
Source :
Biochemical Society Transactions. 32:1107-1109
Publication Year :
2004
Publisher :
Portland Press Ltd., 2004.

Abstract

We have investigated the cellular distribution of p122RhoGAP, a GTPase-activating protein of Rho small GTPase and an activator of phospholipase C-δ1. Immunofluorescence studies demonstrated that endogenous p122 is localized at the tips of actin stress fibres and co-localizes with vinculin in normal rat kidney cells. In immunoprecipitation studies, p122 co-precipitated with vinculin, indicating that p122 is localized at the sites of focal adhesion. We have also shown that the N-terminal half of p122 is responsible for this localization. It is conceivable, therefore, that p122 is involved in the reorganization of the actin cytoskeleton and focal adhesions that regulate cell–substratum adhesion and cell migration.

Details

ISSN :
14708752 and 03005127
Volume :
32
Database :
OpenAIRE
Journal :
Biochemical Society Transactions
Accession number :
edsair.doi.dedup.....0d600434b033b17e8aefa137f103163e
Full Text :
https://doi.org/10.1042/bst0321107