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Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection

Authors :
Haruna Watanabe
Tomomi Takano
Tomoyoshi Doki
Hajime Kusuhara
Source :
The Journal of Veterinary Medical Science
Publication Year :
2021
Publisher :
Japanese Society of Veterinary Science, 2021.

Abstract

Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.

Details

ISSN :
13477439 and 09167250
Volume :
83
Database :
OpenAIRE
Journal :
Journal of Veterinary Medical Science
Accession number :
edsair.doi.dedup.....0d56a5c5d97094614502b5c9b6f93166
Full Text :
https://doi.org/10.1292/jvms.20-0703