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Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
- Source :
- The Journal of Veterinary Medical Science
- Publication Year :
- 2021
- Publisher :
- Japanese Society of Veterinary Science, 2021.
-
Abstract
- Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.
- Subjects :
- Diagnostic methods
Genotype
Cat Diseases
Real-Time Polymerase Chain Reaction
medicine.disease_cause
Feces
Virology
diagnostic method
medicine
Animals
Humans
genogroup
Caliciviridae Infections
General Veterinary
Reverse Transcriptase Polymerase Chain Reaction
business.industry
feline norovirus (FNoV)
Norovirus
Medical practice
Note
Gastroenteritis
Virus detection
Real-time polymerase chain reaction
commercial human kit
Cats
business
Subjects
Details
- ISSN :
- 13477439 and 09167250
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Medical Science
- Accession number :
- edsair.doi.dedup.....0d56a5c5d97094614502b5c9b6f93166
- Full Text :
- https://doi.org/10.1292/jvms.20-0703