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Increased coiling frequency linked to apoptosis in the brain and altered thyroid signaling in zebrafish embryos (Danio rerio) exposed to the PBDE metabolite 6-OH-BDE-47
- Source :
- Chemosphere. 198
- Publication Year :
- 2017
-
Abstract
- Polybrominated diphenyl ethers (PBDEs) are a group of brominated flame retardants that are ubiquitously detected in the environment and associated with adverse health outcomes. 6-OH-BDE-47 is a metabolite of the flame retardant, 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47), and there is increasing concern regarding its developmental neurotoxicity and endocrine disrupting properties. In this study, we report that early life exposure in zebrafish (Danio rerio) embryos to 6-OH-BDE-47 (50 and 100 nM) resulted in higher coiling frequency and significantly increased apoptotic cells in the brain. These effects were partially rescued by overexpression of thyroid hormone receptor β (THRβ) mRNA. Moreover, exposure to 100 nM 6-OH-BDE-47 significantly reduced the number of hypothalamic 5-hydroxytryptamine (5-HT, serotonin)-immunoreactive (5-HT-ir) neurons and the mRNA expression of tryptophan hydroxylase 2 (TPH2). These results indicate that 6-OH-BDE-47 affected thyroid hormone regulation through THRβ and negatively impacted the nervous system, in turn, affecting coiling behavior. Correlations of these endpoints suggest that coiling frequency could be used as an indicator of neurotoxicity in embryos.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Serotonin
Thyroid Hormones
Environmental Engineering
Embryo, Nonmammalian
Health, Toxicology and Mutagenesis
Metabolite
Polybrominated Biphenyls
Danio
Hypothalamus
Thyroid Gland
Apoptosis
010501 environmental sciences
Endocrine Disruptors
01 natural sciences
Article
Thyroid hormone receptor beta
03 medical and health sciences
chemistry.chemical_compound
Polybrominated diphenyl ethers
Internal medicine
medicine
Halogenated Diphenyl Ethers
Environmental Chemistry
Polybrominated Diphenyl Ether
Animals
Coiling
Zebrafish
0105 earth and related environmental sciences
Flame Retardants
Neurons
Thyroid hormone receptor
TPH2
biology
Public Health, Environmental and Occupational Health
Neurotoxicity
Thyroid Hormone Receptors beta
General Medicine
General Chemistry
Tryptophan hydroxylase
medicine.disease
biology.organism_classification
Pollution
030104 developmental biology
Endocrinology
chemistry
Signal Transduction
Subjects
Details
- ISSN :
- 18791298
- Volume :
- 198
- Database :
- OpenAIRE
- Journal :
- Chemosphere
- Accession number :
- edsair.doi.dedup.....0d504a30aab4daeb6919b245cb530b04