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Identification and Characterization of a Versatile Retinoid Response Element (Retinoic Acid Receptor Response Element-Retinoid X Receptor Response Element) in the Mouse Tissue Transglutaminase Gene Promoter
- Source :
- Journal of Biological Chemistry. 271:4355-4365
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- Tissue transglutaminase (transglutaminase type II) is an intracellular protein cross-linking enzyme that accumulates in connective tissue and in cells undergoing apoptosis. Retinoids regulate the transcription of the mouse tissue transglutaminase gene via activation of regulatory elements contained within 4 kilobases of the 5'-end of the gene. Co-transfection studies with retinoid receptor expression vectors in CV-1 cells demonstrated that the mouse tissue transglutaminase promoter is activated by ligand activation of either retinoic acid receptor-retinoid X receptor (RAR.RXR) heterodimers or RXR homodimers. Optimal induction is achieved with retinoid receptor panagonists; partial activation can also be achieved with either RAR-specific or RXR-specific retinoids. Retinoid-dependent activation of the tissue transglutaminase promoter depends on both a proximal regulatory region containing sequences highly conserved between the human and the mouse tissue transglutaminase promoters and a distal region that includes a 30-base pair retinoid response element (mTGRRE1). mTGRRE1 contains three hexanucleotide half-sites (two canonical and one non-canonical) in a DR7/DR5 motif that bind both RAR*RXR heterodimers and RXR homodimers. These studies suggest that retinoid-dependent expression of the mouse tissue transglutaminase gene is mediated by a versatile tripartite retinoid response element located 1.7 kilobases upstream of the transcription start site.
- Subjects :
- Chloramphenicol O-Acetyltransferase
Transcription, Genetic
Receptors, Retinoic Acid
Molecular Sequence Data
Response element
Retinoid receptor
Regulatory Sequences, Nucleic Acid
Biology
Retinoid X receptor
Transfection
Biochemistry
Gene Expression Regulation, Enzymologic
Cell Line
Mice
Retinoids
Liver X receptor beta
Sequence Homology, Nucleic Acid
Chlorocebus aethiops
Animals
Humans
Luciferases
Promoter Regions, Genetic
Molecular Biology
Base Composition
Binding Sites
Transglutaminases
Base Sequence
Retinoid X receptor alpha
3T3 Cells
Cell Biology
Retinoid X receptor gamma
Molecular biology
Introns
DNA-Binding Proteins
Retinoid X Receptors
Retinoic acid receptor alpha
embryonic structures
Retinoid X receptor beta
Transcription Factors
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 271
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....0d47e28acbfbc73c48f7484d21090406