Back to Search
Start Over
Identification of conserved and HLA-A*2402-restricted epitopes in Dengue virus serotype 2
- Source :
- Virus Research. 196:5-12
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- In this study, we set out to identify dengue virus serotype 2 (DENV-2)-specific HLA-A*2402-restricted epitopes and determine the characteristics of T cells generated to these epitopes. We screened the full-length amino-acid sequence of DENV-2 to find potential epitopes using the SYFPEITHI algorithm. Twelve putative HLA-A*2402-binding peptides conserved in hundreds of DENV-2 strains were synthesized, and the HLA restriction of peptides was tested in HLA-A*2402 transgenic mice. Nine peptides (NS4b(228-237), NS2a(73-81), E(298-306), M(141-149), NS4a(96-105), NS4b(159-168), NS5(475-484), NS1(162-171), and NS5(611-620)) induced high levels of peptide-specific IFN-γ-secreting cells in HLA-A*2402 transgenic mice. Apart from IFN-γ, NS4b(228-237-), NS2a(73-81-) and E(298-306)-specific CD8(+) cells produced TNF-α and IL-6 simultaneously, whereas M(141-149-) and NS5(475-484-) CD8(+) cells produced only IL-6. Moreover, splenic mononuclear cells (SMCs) efficiently recognized and killed peptide-pulsed splenocytes. Furthermore, each of nine peptides could be recognized by splenocytes from DENV-2-infected HLA-A*2402 transgenic mice. The SMCs from HLA-A*2402 transgenic mice immunized with nine immunogenic peptides efficiently killed DENV-2-infected splenic monocytes. The present identified epitopes have the potential to be new diagnostic tools for characterization of T-cell immunity in DENV infection and may serve as part of a universal epitope-based vaccine.
- Subjects :
- Serotype
Cancer Research
viruses
Epitopes, T-Lymphocyte
Mice, Transgenic
Dengue virus
Biology
Serogroup
medicine.disease_cause
Peripheral blood mononuclear cell
Epitope
Cell Line
Immunophenotyping
Dengue
Epitopes
Immunity
Virology
medicine
Splenocyte
Animals
Humans
Amino Acid Sequence
HLA-A Antigens
virus diseases
Dengue Virus
Molecular biology
HLA-A
Disease Models, Animal
Infectious Diseases
Cytokines
Female
Immunization
Peptides
Epitope Mapping
CD8
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 01681702
- Volume :
- 196
- Database :
- OpenAIRE
- Journal :
- Virus Research
- Accession number :
- edsair.doi.dedup.....0d2436c92fe20cfac407d97120634665