Absolute recording spectrofluorimeter

An instrument is described that measures fluorescence spectra corrected for variation of detector sensitivity with wavelength and records the results in terms of any desired intensity and wavelength-wavenumber scale. The sensitivity correction function is easily adjusted or replaced by an alternative function. A wavelength-wavenumber facility enables the system to be used for recording in either mode with both prism and grating monochromators. The wavelength range of the present instrument is about 275-628 nm, which is scanned in about 3.3 min.