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High prevalence of clade 8 Escherichia coli O157:H7 isolated from retail meat and butcher shop environment
- Source :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2016
- Publisher :
- Elsevier Science, 2016.
-
Abstract
- Escherichia coli O157:H7 is an enteric pathogen associated with food safety threats and with significant morbidity and mortality worldwide. In Argentina, post-enteric hemolytic uremic syndrome (HUS) is endemic, with >70% of cases associated with E. coli O157 infection. To date the biological basis behind the severity among E. coli O157 infections is unknown. However, single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades, of which clade 8 strains are associated with severe disease cases. The aim of this study was to characterize a collection of 20 STEC O157:H7 strains isolated between 2011 and 2013 from ground beef and different environmental samples from butcher shops of Argentina. All strains harbored the eae, ehxA, fliCH7, efa, iha, and toxB genes, with stx2a/stx2c as the predominant genotype (75%). The XbaI-PFGE analysis showed that the E. coli O157 strains had high genetic diversity. Nine strains were grouped in four XbaI-PFGE clusters, whereas 11 strains showed unique XbaI-PFGE patterns. In contrast, the SNP analysis allowed us to separate the strains in two distinct lineages representing clade 8 (70%) and clade 6 (30%). Our results show the molecular characterization of E. coli O157 strains isolated from ground beef and environmental samples from Argentinean butcher shops.<br />Instituto de Genética Veterinaria
- Subjects :
- 0301 basic medicine
Microbiology (medical)
DNA, Bacterial
Meat
BUTCHER SHOPS
Otras Ciencias Biológicas
030106 microbiology
Argentina
Zoology
Biology
Environment
medicine.disease_cause
Clade 8
Escherichia coli O157
Microbiology
Polymorphism, Single Nucleotide
Ciencias Biológicas
purl.org/becyt/ford/1 [https]
03 medical and health sciences
Molecular typing
fluids and secretions
Genetics
medicine
Environmental Microbiology
Animals
Shop (environment)
Clade
purl.org/becyt/ford/1.6 [https]
Molecular Biology
Escherichia coli
Ecology, Evolution, Behavior and Systematics
Butcher
Phylogeny
CLADE 8
ARGENTINA
ENVIRONMENT
High prevalence
MEAT
Ciencias Veterinarias
Butcher shops
Molecular Typing
030104 developmental biology
Infectious Diseases
H7 [O157]
Cattle
O157:h7
CIENCIAS NATURALES Y EXACTAS
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Accession number :
- edsair.doi.dedup.....0d1718dca2eef2524ab9e40fed6a9f9a