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Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A
- Source :
- The Journal of Cell Biology
- Publication Year :
- 2010
-
Abstract
- In addition to its function as an Arp2/3 complex subunit, Arp1cb interacts with and stimulates Aurora A at centrosomes, functioning in cell cycle progression.<br />Here we provide evidence in support of an inherent role for Arpc1b, a component of the Arp2/3 complex, in regulation of mitosis and demonstrate that its depletion inhibits Aurora A activation at the centrosome and impairs the ability of mammalian cells to enter mitosis. We discovered that Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on threonine 21, and expression of Arpc1b but not a nonphosphorylatable Arpc1b mutant in mammalian cells leads to Aurora A kinase activation and abnormal centrosome amplification in a Pak1-independent manner. Together, these findings reveal a new function for Arpc1b in centrosomal homeostasis. Arpc1b is both a physiological activator and substrate of Aurora A kinase and these interactions help to maintain mitotic integrity in mammalian cells.
- Subjects :
- Aurora inhibitor
Aurora A kinase
Enzyme Activators
Mitosis
Centrosome cycle
macromolecular substances
Biology
Protein Serine-Threonine Kinases
Article
Actin-Related Protein 2-3 Complex
03 medical and health sciences
0302 clinical medicine
Aurora Kinases
Tubulin
Cell Line, Tumor
Humans
Phosphorylation
Research Articles
030304 developmental biology
Centrosome
0303 health sciences
Activator (genetics)
Cell Biology
3. Good health
Cell biology
enzymes and coenzymes (carbohydrates)
Spindle checkpoint
030220 oncology & carcinogenesis
embryonic structures
Mutation
biology.protein
biological phenomena, cell phenomena, and immunity
Subjects
Details
- ISSN :
- 15408140
- Volume :
- 190
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of cell biology
- Accession number :
- edsair.doi.dedup.....0d1012ec16618c24e4c0af8694445a6a