Quantitative proteomic analysis of the effects of a GalNAc/Man-specific lectin CSL on yeast cells by label-free LC–MS

Authors :: Oleg V. Chernikov
Wei Li
Qiancheng Zhao
Changqing Tong
Shuai Liu
P. A. Luk’yanov
Li Linge
Source :: International Journal of Biological Macromolecules. 85:530-538
Publication Year :: 2016
Publisher :: Elsevier BV, 2016.
Abstract: A Ca(2+)-dependent GalNAc/Man-specific lectin (CSL) from Cyclina sinensis was isolated, and its stimulatory action was characterized in yeast. CSL showed a potent effect on the production of ethanol by Saccharomyces cerevisiae. In this work, the changes in the protein expression profiles of S. cerevisiae after 24h of incubation with CSL were analyzed using label-free quantitative proteomics. A total of 1410 proteins were identified, but only 117 proteins showed significant differences in normalized volume (p

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