Back to Search
Start Over
MicroRNA-27a-3p directly targets FosB to regulate cell proliferation, apoptosis, and inflammation responses in immunoglobulin a nephropathy
- Source :
- Biochemical and Biophysical Research Communications. 529:1124-1130
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Immunoglobulin A nephropathy (IgAN) constitutes the most common primary glomerulonephritis worldwide; however, the exact pathogenesis of IgAN is unknown. Previous genome-wide analysis of microRNA (miRNA) expression in the kidney has confirmed that miRNAs are closely related to the pathological changes of IgAN. Accordingly, in this study we found that miR-27a-3p is upregulated in IgAN kidney tissues in addition to human podocytes and tubule epithelial HK2 but not mesangial cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT), flow cytometry, real-time polymerase chain reaction, western blot, and enzyme-linked immunosorbent assays were used to verify the regulatory effects of miR-27a-3p and its inhibition on cell proliferation, apoptosis, and release of inflammatory factors in podocytes and HK2 cells. The target genes of miR-27a-3p were predicted using bioinformatics software; the identity of FosB as a target gene of miR-27a-3p was confirmed by luciferase report assay and western blot. Overall, our findings demonstrated that miR-27a-3p regulates cell apoptosis, cell proliferation, and the release of inflammatory cytokines of human podocytes and HK2 cells by directly targeting FosB. Our results therefore suggested that miR-27a-3p might be associated with the pathophysiology of IgAN and may represent a potential target for further studies related to IgAN mechanism or therapeutics.
- Subjects :
- 0301 basic medicine
Biophysics
Apoptosis
Inflammation
Biology
urologic and male genital diseases
Biochemistry
Cell Line
Proinflammatory cytokine
03 medical and health sciences
0302 clinical medicine
Western blot
microRNA
medicine
Humans
Molecular Biology
Cell Proliferation
Base Sequence
medicine.diagnostic_test
Cell growth
Glomerulonephritis, IGA
Glomerulonephritis
Cell Biology
medicine.disease
MicroRNAs
030104 developmental biology
Gene Expression Regulation
030220 oncology & carcinogenesis
Cancer research
Cytokines
Inflammation Mediators
medicine.symptom
Proto-Oncogene Proteins c-fos
FOSB
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 529
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....0cf670b6b52b9e164f94fb84a2637dd2