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Transcription factor AP1 binds the functional region of the promoter and regulates gene expression of human PPARdelta in LoVo cell
- Source :
- Tumor Biology. 34:3619-3625
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Peroxisome proliferator-activated receptor δ gene (PPARδ) is correlated with carcinogenesis of colorectal cancer, but the regulation of its gene transcription remains unclear. We herein report that AP1 binds the promoter and regulates PPARδ gene expression. With a luciferase reporter system, we identified a functional promoter region of 30 bp of PPARδ gene by deletion and electrophoretic mobility shift assays (EMSA). Using site-directed mutagenesis and decoy analyses, we demonstrated that AP1 bound the functional transcriptional factor binding site in a region extending from -176 to -73 of the PPARδ promoter, which was confirmed using EMSA and supershift assays. Consequently, inhibition of the AP1 binding site led to decreased PPARδ mRNA. Our study demonstrated that AP1 is the transcriptional factor that contributes to PPARδ expression in LoVo cells.
- Subjects :
- Electrophoretic Mobility Shift Assay
Biology
Transfection
Cell Line, Tumor
Gene expression
Humans
Electrophoretic mobility shift assay
PPAR delta
Binding site
Luciferases
Promoter Regions, Genetic
Transcription factor
Gene
Anthracenes
Cell Nucleus
Regulation of gene expression
Binding Sites
Base Sequence
integumentary system
Reverse Transcriptase Polymerase Chain Reaction
Promoter
General Medicine
Molecular biology
Transcription Factor AP-1
AP-1 transcription factor
Gene Expression Regulation
Colonic Neoplasms
Mutagenesis, Site-Directed
Protein Binding
Subjects
Details
- ISSN :
- 14230380 and 10104283
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Tumor Biology
- Accession number :
- edsair.doi.dedup.....0cdd964194d018935ba11e63df55a283