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Anti-inflammatory and Mineralization Effects of ProRoot MTA and Endocem MTA in Studies of Human and Rat Dental Pulps In Vitro and In Vivo
- Source :
- Journal of Endodontics. 44:1534-1541
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Introduction Few studies have reported direct pulp capping in inflamed pulp conditions. The purpose of this study was to investigate the in vitro and in vivo responses of dental pulp during direct pulp capping using various pulp capping materials in inflamed conditions. Methods Human dental pulp cells were treated with lipopolysaccharide (LPS) and cultured with Dycal (Dentsply Caulk, Milford, DE), ProRoot MTA (Dentsply Maillefer, Ballaigues, Switzerland), and Endocem MTA (Maruchi, Wonju, South Korea). The expressions of interleukin (IL)-1β, IL-6, dentin matrix protein 1, and dentin sialophosphoprotein were analyzed through real-time polymerase chain reaction. The maxillary molars of Sprague-Dawley rats were exposed for 2 days. The exposed pulps were capped with Dycal, ProRoot MTA, and Endocem MTA and sealed with resin-modified glass ionomer followed by histologic and immunohistochemical analyses. Results The expression of IL-1β and IL-6 was increased with LPS and decreased by Dycal, ProRoot MTA, and Endocem MTA. Dentin matrix protein 1 and dentin sialophosphoprotein levels were decreased with LPS and increased after treatment with pulp capping materials.In the in vivo study, inflammation associated with Dycal was higher than that associated with ProRoot MTA and Endocem MTA at week 1, without any significant difference between the 2. At 4 weeks, inflammation was decreased, and mineralization was increased compared with week 1 in all 3 of the materials. At week 1, IL-6 immunoreactivity was strongly expressed. Dycal exhibited stronger immunoreactivity than ProRoot MTA and Endocem MTA. However, the immunoreactivity was decreased in all groups at week 4. Conclusions Successful direct pulp capping requires more effective pulp capping materials for the treatment of inflamed pulps.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Molar
medicine.drug_class
Interleukin-1beta
Anti-Inflammatory Agents
Glass ionomer cement
Dental Pulp Capping
Anti-inflammatory
Calcium Hydroxide
Rats, Sprague-Dawley
Root Canal Filling Materials
Andrology
03 medical and health sciences
Calcification, Physiologic
0302 clinical medicine
stomatognathic system
Dentin sialophosphoprotein
In vivo
Dentin
medicine
Animals
Humans
General Dentistry
Cells, Cultured
Dental Pulp
Minerals
Interleukin-6
Chemistry
030206 dentistry
Rats
Pulp capping
stomatognathic diseases
030104 developmental biology
medicine.anatomical_structure
Pulp (tooth)
Inflammation Mediators
Pulp Capping and Pulpectomy Agents
Subjects
Details
- ISSN :
- 00992399
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Endodontics
- Accession number :
- edsair.doi.dedup.....0cdbe20184d3eb1d1094a4ba48800f11