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Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine
- Source :
- Analytical and Bioanalytical Chemistry. 384:1254-1258
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w), and 10 mmol L(-1) sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes.
- Subjects :
- Analyte
Time Factors
Sodium
Molecular Conformation
chemistry.chemical_element
Sensitivity and Specificity
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Electrokinetic phenomena
Pulmonary surfactant
Capillary Electrochromatography
Animals
Microemulsion
Medicine, Chinese Traditional
Sodium dodecyl sulfate
Cinobufagin
Heptane
Chromatography
Temperature
Reproducibility of Results
Sodium Dodecyl Sulfate
Stereoisomerism
Hydrogen-Ion Concentration
Bufanolides
Pharmaceutical Preparations
chemistry
Amphibian Venoms
Emulsions
Anura
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 384
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....0cc5ec25ceff71e3dd5d2945e380df81