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Purified Mouse Schwann Cells: Mitogenic Effects of Fetal Calf Serum and Fibroblast Growth Factor
- Source :
- Developmental Neuroscience. 5:77-91
- Publication Year :
- 1982
- Publisher :
- S. Karger AG, 1982.
-
Abstract
- We present an in vitro bioassay protocol for the detailed examination of mitogenic influences on purified mouse Schwann cells. This involves measurements of (i) increases in Schwann cell number per culture, (ii) incorporation of radiolabeled thymidine per culture, and (iii) the proportion of total Schwann cells that exhibit a labeled nucleus by autoradiography. Using this standard protocol we show that the mitogenic efforts of fetal calf serum must be distinguished from its effects on cell retention to the culture substratum, and preparations of mouse brain or pituitary fibroblast growth factor but not mouse epidermal growth factor are very potent mouse Schwann cell mitogens. These mitogenic effects of serum and fibroblast growth factor have not been reported for rat Schwann cells.
- Subjects :
- medicine.medical_specialty
Cell division
Cell
Schwann cell
Fibroblast growth factor
Mice
chemistry.chemical_compound
Developmental Neuroscience
Epidermal growth factor
Internal medicine
medicine
Animals
Cells, Cultured
Mice, Inbred ICR
Fetus
Epidermal Growth Factor
Chemistry
DNA
Fetal Blood
Molecular biology
In vitro
Fibroblast Growth Factors
medicine.anatomical_structure
Endocrinology
nervous system
Neurology
Schwann Cells
Mitogens
Peptides
Thymidine
Dialysis
Cell Division
Subjects
Details
- ISSN :
- 14219859 and 03785866
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Developmental Neuroscience
- Accession number :
- edsair.doi.dedup.....0cc2cfd5851c7a4b6895f1933ecb39ee