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A host-specific function is required for ligation of a wide variety of ribozyme-processed RNAs
- Source :
- Proceedings of the National Academy of Sciences. 97:424-429
- Publication Year :
- 2000
- Publisher :
- Proceedings of the National Academy of Sciences, 2000.
-
Abstract
- Hepatitis δ virus (HDV) replicates its circular RNA genome via a rolling circle mechanism. During this process, cis-acting ribozymes cleave adjacent upstream sequences and thereby resolve replication intermediates to unit-length RNA. The subsequent ligation of these 5′OH and 2′,3′-cyclic phosphate termini to form circular RNA is an essential step in the life cycle of the virus. Here we present evidence for the involvement of a host activity in the ligation of HDV RNA. We used both HDV and hammerhead ribozymes to generate a panel of HDV and non-HDV RNA substrates that bear 5′ hydroxyl and 2′,3′- cyclic phosphate termini. We found that ligation of these substrates occurred in host cells, but not in vitro or in Escherichia coli . The host-specific ligation activity was capable of joining RNA in both bimolecular and intramolecular reactions and functioned in a sequence-independent manner. We conclude that mammalian cells contain a default pathway that efficiently circularizes ribozyme processed RNAs. This pathway could be exploited in the delivery of stable antisense and decoy RNA to the nucleus.
- Subjects :
- viruses
Molecular Sequence Data
Virus
Cell Line
Viral Proteins
Circular RNA
RNA Precursors
Humans
RNA, Catalytic
Ligase ribozyme
Multidisciplinary
Base Sequence
biology
Ribozyme
RNA
DNA-Directed RNA Polymerases
Biological Sciences
Blotting, Northern
Molecular biology
Cell biology
Polynucleotide Ligases
Rolling circle replication
Mutation
biology.protein
Nucleic Acid Conformation
RNA, Viral
Hepatitis Delta Virus
Ligation
Hairpin ribozyme
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....0cbccc2b48cb80991fb8213ef7a8075d