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Cicadidae Periostracum Attenuates Atopic Dermatitis Symptoms and Pathology via the Regulation of NLRP3 Inflammasome Activation
- Source :
- Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2021 (2021)
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Atopic dermatitis (AD) is a multifactorial inflammatory skin disease of complex etiology. Despite its increasing prevalence, treatment for AD is still limited. Crude drugs, including herbal extracts or natural resources, are being used to treat AD symptoms, with minimum side effects. Cicadidae Periostracum (CP), derived from the slough of insects belonging to the family Cicadidae, is a commonly used crude drug in traditional Asian medicine to treat/control epilepsy, shock, and edema. However, the effect of CP on AD-like skin lesions is unknown. In this study, we examined the effect of a CP water extract on AD disease development in vivo, using a house dust mite-induced AD mouse model, and in vitro, using HaCaT keratinocytes and a 3D human skin equivalent system. Importantly, CP administration alleviated house dust mite-induced AD-like symptoms, suggested by the quantified dermatitis scores, animal scratching behaviors, skin moisture retention capacity, and skin lesion and ear thickness. Furthermore, histopathological analysis demonstrated that CP decreased intralesional mast cell infiltration. In addition, CP treatments decreased the systemic levels of immunoglobulin E, histamine, and thymic stromal lymphopoietin (TSLP) and the local mRNA expression of TSLP and several Th1/Th2 cytokines. Our data suggest that these effects were mediated by the inhibition of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome activation. In vivo and in vitro CP treatments resulted in the downregulation of inflammasome components, such as ASC and cleaved caspase-1, as well as related mediators such as IL-1β and reactive oxygen species. Collectively, our results suggest that CP is a potential therapeutic agent for AD, controlling inflammatory responses through the suppression of NLRP3 inflammasome activation.
- Subjects :
- Male
0301 basic medicine
Aging
Thymic stromal lymphopoietin
Article Subject
Inflammasomes
Human skin
Complex Mixtures
Immunoglobulin E
Biochemistry
Dermatitis, Atopic
Hemiptera
Mice
03 medical and health sciences
chemistry.chemical_compound
Th2 Cells
0302 clinical medicine
In vivo
NLR Family, Pyrin Domain-Containing 3 Protein
medicine
Animals
QH573-671
integumentary system
biology
business.industry
Inflammasome
Cell Biology
General Medicine
Atopic dermatitis
Th1 Cells
medicine.disease
Disease Models, Animal
HaCaT
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Immunology
biology.protein
Cytokines
Cytology
business
Histamine
Research Article
medicine.drug
Subjects
Details
- ISSN :
- 19420994 and 19420900
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....0cb635b9b6fcd337aac422c29dd4ec38