Back to Search
Start Over
Isoproterenol accelerates apoptosis through the over-expression of the sodium/calcium exchanger in HeLa cells
- Source :
- General physiology and biophysics. 32:311-323
- Publication Year :
- 2014
- Publisher :
- AEPress, s.r.o., 2014.
-
Abstract
- Apoptosis induction causes over-expression of the Na + /Ca 2+ exchanger of type 1 (NCX1) in the HeLa cell line. During induction of apoptosis and in the presence of isoproterenol hydrochlo- ride (I; β-adrenergic agonist), increase in the NCX1 is even more pronounced. Anti-apoptotic Bcl-2 mRNA and protein is markedly reduced during apoptosis and in the presence of I, which causes a rapid increase in the Bax/Bcl-2 ratio. During apoptosis induction by apoptosis inducing kit (A), both with and without I, the active form of caspase-3, which is the executive enzyme in apoptosis, becomes visible on Western blots. Silencing NCX1 resulted in the reversal of the Bax/Bcl-2 ratio, it prevented a decrease in mitochondrial membrane potential compared to the AI group and it decreased the level of AI-induced apoptosis in HeLa cells. Based on the experiments with single apoptotic inducers camptothecin, cycloheximide and dexamethasone, it might be proposed that potentiated apoptotic effect in I-treated cells is due to the inhibition of nuclear topoisomerase. As illustrated in immunofluorescence and Western blot analysis, calnexin increased significantly during induction of the apoptosis in the presence of I. In addition, further decrease in sarco/endoplasmic ATPase 2 (SERCA2), decrease in reticular calcium and mitochondrial membrane potential was observed, which suggests development of the endoplasmic reticulum (ER) stress. Based on these results, we propose that I further enhanced NCX1 expression in apoptotic cells through the development of ER stress.
- Subjects :
- Physiology
Biophysics
Apoptosis
Cycloheximide
Biology
Sodium-Calcium Exchanger
HeLa
chemistry.chemical_compound
medicine
Humans
RNA, Messenger
bcl-2-Associated X Protein
Sodium-calcium exchanger
Caspase 3
Endoplasmic reticulum
Isoproterenol
General Medicine
Adrenergic beta-Agonists
biology.organism_classification
Molecular biology
Cell biology
Blot
Gene Expression Regulation
Proto-Oncogene Proteins c-bcl-2
chemistry
Unfolded protein response
Camptothecin
HeLa Cells
medicine.drug
Subjects
Details
- ISSN :
- 13384325
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- General physiology and biophysics
- Accession number :
- edsair.doi.dedup.....0c8d939965e1892eb71e58f89c66b257
- Full Text :
- https://doi.org/10.4149/gpb_2013044