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Standardized two-step testing of antibody activity in COVID-19 convalescent plasma

Authors :
Pavlo Gilchuk
Isaac Thomsen
Sandra Yoder
Eric Brady
James D. Chappell
Laura J. Stevens
Mark R. Denison
Rachel E. Sutton
Rita E. Chen
Laura A. VanBlargan
Naveenchandra Suryadevara
Seth J. Zost
Jonathan Schmitz
Jill M. Pulley
Michael S. Diamond
Jillian P. Rhoads
Gordon R. Bernard
Wesley H. Self
Todd W. Rice
Allison P. Wheeler
James E. Crowe
Robert H. Carnahan
Source :
iScience, iScience, Vol 25, Iss 1, Pp 103602-(2022)
Publication Year :
2021
Publisher :
The Authors., 2021.

Abstract

The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.<br />Graphical abstract<br />Immunology; Virology

Details

Language :
English
ISSN :
25890042
Volume :
25
Issue :
1
Database :
OpenAIRE
Journal :
iScience
Accession number :
edsair.doi.dedup.....0c751360d279375267685077368886ed