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Standardized two-step testing of antibody activity in COVID-19 convalescent plasma
- Source :
- iScience, iScience, Vol 25, Iss 1, Pp 103602-(2022)
- Publication Year :
- 2021
- Publisher :
- The Authors., 2021.
-
Abstract
- The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated viral neutralization, and there is a need for standardized quantitative neutralization assays. We report a high-throughput two-step profiling approach for identifying neutralizing convalescent plasma. Screening and downselection for serum antibody binding to the receptor-binding domain are followed by quantitative neutralization testing using a chimeric vesicular stomatitis virus expressing spike protein of SARS-CoV-2 in a real-time cell analysis assay. This approach enables a predictive screening process for identifying plasma units that neutralize SARS-CoV-2. To calibrate antibody neutralizing activity in serum from convalescent plasma donors, we introduce a neutralizing antibody standard reagent composed of two human antibodies that neutralize SARS-CoV strains, including SARS-CoV-2 variants of concern. Our results provide a framework for establishing a standardized assessment of antibody-based interventions against COVID-19.<br />Graphical abstract<br />Immunology; Virology
- Subjects :
- Multidisciplinary
Science
Virology
Immunology
Article
Subjects
Details
- Language :
- English
- ISSN :
- 25890042
- Volume :
- 25
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- iScience
- Accession number :
- edsair.doi.dedup.....0c751360d279375267685077368886ed