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Establishment and characterization of fetal equine kidney and lung cells with extended lifespan. Susceptibility to equine gammaherpesvirus infection and transfection efficiency
- Source :
- In vitro cellulardevelopmental biology. Animal. 52(8)
- Publication Year :
- 2016
-
Abstract
- Due to the slow growth of equine gammaherpesviruses, isolation of these viruses requires cells that can be propagated long term and show clear cytopathy following infection. Equine cell lines with extended lifespan were established from primary cells originating from equine fetal kidney and lung by transfecting the cells with the retroviral vector LXSN116E6E7 containing the human papilloma virus oncogenes 16 E6 and E7. The transfected equine kidney cell line and equine lung cell line can be propagated for more than 40 passages, whereas the corresponding primary cells only for 10–12 passages. The primary cells and the derived cell lines can be infected with equine gammaherpesvirus 2 (EHV-2) with similar efficiency. However EHV-5 can be grown to a substantially higher titer in the kidney cell line than their primary counterpart, with cytopathic effect visible three days earlier than in the primary cells. Due to rapid cell growth the lung cell line is difficult to use for virus production. The kidney cell line was four times more susceptible to transfection as compared to the primary kidney cells. On the other hand no difference was between the lung cell line and the primary lung cells in transfection efficiency. The cell lines can be a valuable tool for investigating gammaherpesviruses, and possibly other viruses infecting horses.
- Subjects :
- 0301 basic medicine
040301 veterinary sciences
viruses
Biology
Kidney
Transfection
Fetal Kidney
Virus
Viral vector
Cell Line
0403 veterinary science
03 medical and health sciences
Gammaherpesvirinae
Animals
Humans
Horses
Lung
Cytopathic effect
Cell growth
fungi
04 agricultural and veterinary sciences
Cell Biology
General Medicine
Herpesviridae Infections
Virology
030104 developmental biology
Cell culture
Disease Susceptibility
Stem cell
Developmental Biology
Subjects
Details
- ISSN :
- 1543706X
- Volume :
- 52
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- In vitro cellulardevelopmental biology. Animal
- Accession number :
- edsair.doi.dedup.....0c682b8598830ad196c7fe3bddb384a1