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Immunoreactive peptide maps of SARS-CoV-2
- Source :
- Communications Biology, Vol 4, Iss 1, Pp 1-7 (2021), Communications Biology
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Serodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity among the human coronaviruses (HCoVs): SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. Here we report the identification of humoral immune responses to SARS-CoV-2 peptides that may enable discrimination between exposure to SARS-CoV-2 and other HCoVs. We used a high-density peptide microarray and plasma samples collected at two time points from 50 subjects with SARS-CoV-2 infection confirmed by qPCR, samples collected in 2004–2005 from 11 subjects with IgG antibodies to SARS-CoV-1, 11 subjects with IgG antibodies to other seasonal human coronaviruses (HCoV), and 10 healthy human subjects. Through statistical modeling with linear regression and multidimensional scaling we identified specific peptides that were reassembled to identify 29 linear SARS-CoV-2 epitopes that were immunoreactive with plasma from individuals who had asymptomatic, mild or severe SARS-CoV-2 infections. Larger studies will be required to determine whether these peptides may be useful in serodiagnostics.<br />Mishra, Huang et al. identify 29 linear SARS-CoV-2 epitopes that are immunoreactive with the plasma from individuals who had asymptomatic, mild, or severe SARS-CoV-2 infections. This study suggests the possibility of using these peptides to discriminate the exposure to SARS-CoV-2 and other human coronaviruses.
- Subjects :
- 0301 basic medicine
Proteome
QH301-705.5
viruses
Medicine (miscellaneous)
Peptide
Asymptomatic
Peptide Mapping
Article
Epitope
Immunoglobulin G
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Epitopes
0302 clinical medicine
Immune system
Chiroptera
medicine
Animals
Humans
Amino Acid Sequence
Biology (General)
skin and connective tissue diseases
Peptide sequence
chemistry.chemical_classification
biology
SARS-CoV-2
Infectious-disease diagnostics
fungi
COVID-19
virus diseases
respiratory tract diseases
body regions
030104 developmental biology
chemistry
Viral infection
Immunology
biology.protein
Peptide microarray
Antibody
medicine.symptom
Infection
General Agricultural and Biological Sciences
Peptides
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 23993642
- Volume :
- 4
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Communications Biology
- Accession number :
- edsair.doi.dedup.....0c65ee44fa648105fb244d9f91f7d9e5