Back to Search
Start Over
Evaluation of Reliability of FISH Versus Brightfield Dual-probe In Situ Hybridization (BDISH) for Frontline Assessment of HER2 Status in Breast Cancer Samples in a Community Setting
- Source :
- American Journal of Surgical Pathology. 36:1489-1496
- Publication Year :
- 2012
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2012.
-
Abstract
- Aims: To evaluate the reliability of novel brightfield microscopy–based dual in situ hybridization (BDISH) methods for frontline HER2 status analysis in selected suboptimally preserved breast cancer tissue samples reflecting of the worst scenario in a community. Methods and Results: A total of 320 morphologically poorly preserved breast invasive ductal carcinomas from the archives of 2 tertiary institutions in Brazil were selected for a tissue microarray–based analysis. 4B5 antibody was used for immunohistochemistry. Fluorescence in situ hybridization (FISH), DuoCISH, ZytoDot CISH, and silver in situ hybridization (SISH) were performed and compared. The highest agreement was observed between SISH and FISH. In addition, SISH was easier to assess in both amplified and nonamplified cases when compared with the other chromogenic methods, due to the sharpness of its dots. DuoCISH produced false-positive results, associated with thicker ill-defined dots, causing poor distinction between nonamplification and low amplification. ZytoDot CISH showed lower sensitivity, with increased frequency of false-positive results. Conclusions: SISH is the most reliable of the BDISH methods, with sensitivity and specificity highly comparable with FISH. It is also less deleterious than other BDISH methods, producing signals that were more distinct and therefore more readily analyzable even in poorly preserved tissue.
- Subjects :
- Adult
Pathology
medicine.medical_specialty
Receptor, ErbB-2
Breast Neoplasms
In situ hybridization
Biology
Pathology and Forensic Medicine
Tertiary Care Centers
Breast cancer
medicine
Humans
Community Health Services
CISH
In Situ Hybridization
In Situ Hybridization, Fluorescence
Aged
Neoplasm Staging
Tissue microarray
medicine.diagnostic_test
Carcinoma, Ductal, Breast
Reproducibility of Results
Middle Aged
medicine.disease
Immunohistochemistry
Tissue Array Analysis
Dual probe
Fish
Female
Surgery
Tissue Preservation
Anatomy
Artifacts
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 01475185
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- American Journal of Surgical Pathology
- Accession number :
- edsair.doi.dedup.....0c5360d27b400b94c1ee594cc6ed9027