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Sustainable extraction of proteins and bioactive substances from pomegranate peel (Punica granatum L.) using pressurized liquids and deep eutectic solvents
- Source :
- e_Buah Biblioteca Digital Universidad de Alcalá, instname, Universidad de Alcalá (UAH)
- Publication Year :
- 2020
-
Abstract
- Pomegranate peel is a source of proteins, bioactive peptides, and phenolic compounds. The simultaneous extraction of these compounds required the use of polluting solvents and reagents that are non-suitable. This work targets the development of green methodologies based on pressurized liquids (PLE) or deep eutectic solvents (DES) for the extraction of these compounds. Extracts were digested with different proteolytic enzymes and different functionalities (antioxidant, hypocholesterolemia, and antihypertensive capacities) were evaluated. Highly antioxidant and hypocholesterolemic extracts and hydrolysates were obtained using PLE while high antihypertensive capacity was observed in the hydrolysates from proteins extracted using DES. Peptides and polyphenols were identified by HPLC-ESI-Q-TOF/MS. Higher amounts of peptides were shown in hydrolysates from DES extracts while hydrolysates from PLE extracts presented higher amounts of phenolic compounds. Some peptides were assigned to proteins from Punica granatum. Both green methods improved the extraction of bioactive compounds from pomegranate peel compared to the non-sustainable method. Industrial relevance The development of green methodologies which employ sustainable solvents such as pressurized liquids (PLE) and deep eutectic solvents (DES) allows extracting proteins and bioactive compounds from pomegranate peel. In addition, these solvents improve the extraction of health beneficial compounds compared to the non-sustainable and polluting solvents. Therefore, they could be used for the development of nutraceuticals and functional foods or even in medicinal, cosmetic, and pharmaceutical preparations.
- Subjects :
- Polyphenol
Antioxidant
medicine.medical_treatment
Pressurized liquid extraction (PLE)
Industrial and Manufacturing Engineering
Hydrolysate
0404 agricultural biotechnology
Nutraceutical
medicine
Deep eutectic solvents (DES)
Chromatography
biology
Mass spectrometry
Chemistry
Extraction (chemistry)
Proteolytic enzymes
04 agricultural and veterinary sciences
General Chemistry
Química
biology.organism_classification
040401 food science
Pomegranate peel
Reagent
Punica
Peptide
Food Science
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- e_Buah Biblioteca Digital Universidad de Alcalá, instname, Universidad de Alcalá (UAH)
- Accession number :
- edsair.doi.dedup.....0c514800ce298baa22f06ab5fceeb874