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Correlating blood-based DNA methylation markers and prostate cancer risk in African-American men
- Source :
- PLoS ONE, Vol 13, Iss 9, p e0203322 (2018)
- Publication Year :
- 2018
-
Abstract
- The objective of this work was to investigate the clinical significance of promoter gene DNA methylation changes in whole blood from African-American (AA) men with prostate cancer (PCa). We used high throughput pyrosequencing analysis to quantify percentage DNA methylation levels in a panel of 8 genes (RARβ2, TIMP3, SPARC, CDH13, HIN1, LINE1, CYB5R2 and DRD2) in blood DNA obtained from PCa and non-cancerous controls cases. Correlations of methylation status and various clinicopathological features were evaluated. Six genes tested achieved significant difference in DNA methylation levels between the PCa compared to control cases (P < 0.05). The TIMP3 loci demonstrated significant correlation of DNA methylation with age for all cases analyzed (p < 0.05). We observed an inverse correlation between CDH13 methylation (p = 0.045; r = -0.21) and serum vitamin D level whereas TIMP3 methylation (p = 0.021; r = -0.24) and DRD2 methylation (p = 0.056; r = -0.201) showed inverse correlation with supplementary vitamin D in the cancer cases. We also observed a direct correlation between methylation of RARβ2 (p = 0.0036; r = 0.293) and SPARC (p = 0.0134; r = 0.20) loci with PSA level in the controls but not the cancer cases. In addition, alcohol cases significantly correlated with higher RARβ2 methylation (p = 0.0314) in comparison with non-alcohol cases. Furthermore, we observed an inverse correlation of DRD2 methylation (p = 0.0349; r = -0.343) and Gleason score. Our data suggests that promoter methylation occurred more frequently in the blood of AA PCa and is associated with various clinicopathological features in AA men with PCa.
- Subjects :
- 0301 basic medicine
Adult
Genetic Markers
Male
Receptors, Retinoic Acid
lcsh:Medicine
Biology
Andrology
03 medical and health sciences
Prostate cancer
0302 clinical medicine
Risk Factors
medicine
Vitamin D and neurology
Biomarkers, Tumor
Humans
Osteonectin
Vitamin D
lcsh:Science
Promoter Regions, Genetic
Whole blood
Aged
Tissue Inhibitor of Metalloproteinase-3
Multidisciplinary
Receptors, Dopamine D2
Tumor Suppressor Proteins
lcsh:R
Cancer
Prostatic Neoplasms
Promoter
Methylation
DNA Methylation
Middle Aged
medicine.disease
Cadherins
Black or African American
030104 developmental biology
Genetic marker
030220 oncology & carcinogenesis
Case-Control Studies
DNA methylation
Cytokines
lcsh:Q
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....0c4610cd4c8bbddf1ca4c02815a5f0c1