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Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 110(24)
- Publication Year :
- 2013
-
Abstract
- Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number of primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF–induced cells with a limited life span are the most common source. We report here a simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator of transcription 5-dependent macrophages (Max Planck Institute cells) from mouse fetal liver, which reflect the innate immune characteristics of alveolar macrophages. Max Planck Institute cells are exquisitely sensitive to selected microbial agents, including bacterial LPS, lipopeptide, Mycobacterium tuberculosis, cord factor, and adenovirus and mount highly proinflammatory but no anti-inflammatory IL-10 responses. They show a unique pattern of innate responses not yet observed in other mononuclear phagocytes. This includes differential LPS sensing and an unprecedented regulation of IL-1α production upon LPS exposure, which likely plays a key role in lung inflammation in vivo. In conclusion, Max Planck Institute cells offer an useful tool to study macrophage biology and for biomedical science.
- Subjects :
- Lipopolysaccharides
Cellular differentiation
Inflammation
Bone Marrow Cells
Mice, Transgenic
Biology
Proinflammatory cytokine
Mice
Phagocytosis
Interleukin-1alpha
Macrophages, Alveolar
medicine
STAT5 Transcription Factor
Macrophage
Animals
Humans
Propionibacterium acnes
Cells, Cultured
Cell Proliferation
Oligonucleotide Array Sequence Analysis
Mice, Knockout
Mice, Inbred BALB C
Multidisciplinary
Innate immune system
Cord factor
Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
Cell Differentiation
Mycobacterium tuberculosis
Molecular biology
Cell biology
Mice, Inbred C57BL
Granulocyte macrophage colony-stimulating factor
PNAS Plus
STAT protein
Cytokines
medicine.symptom
Transcriptome
medicine.drug
Subjects
Details
- ISSN :
- 10916490
- Volume :
- 110
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....0c18401896ee99c5597f9f1376613af3