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The Effect of Organophosphate Exposure on Neuronal Cell Coenzyme Q10 Status
- Source :
- Neurochemical Research
- Publication Year :
- 2020
- Publisher :
- Springer US, 2020.
-
Abstract
- Organophosphate (OP) compounds are widely used as pesticides and herbicides and exposure to these compounds has been associated with both chronic and acute forms of neurological dysfunction including cognitive impairment, neurophysiological problems and cerebral ataxia with evidence of mitochondrial impairment being associated with this toxicity. In view of the potential mitochondrial impairment, the present study aimed to investigate the effect of exposure to commonly used OPs, dichlorvos, methyl-parathion (parathion) and chloropyrifos (CPF) on the cellular level of the mitochondrial electron transport chain (ETC) electron carrier, coenzyme Q10 (CoQ10) in human neuroblastoma SH-SY5Y cells. The effect of a perturbation in CoQ10 status was also evaluated on mitochondrial function and cell viability. A significant decreased (P 10 status, with levels of this isoprenoid being decreased by 72% (P 10 supplementation (5 μM) was able to restore cellular CoQ10 status and CS activity to control levels following OP treatment, complex II+III activity was only restored to control levels in neuronal cells exposed to dichlorvos (50 µM). However, post supplementation with CoQ10, complex II+III activity significantly increased by 33% (P 10 supplemented cells. In conclusion, the results of this study have indicated evidence of neuronal cell CoQ10 deficiency with associated mitochondrial dysfunction following OP exposure. Although CoQ10 supplementation was able to ameliorate OP induced deficiencies in CS activity, ETC complex II+III activity appeared partially refractory to this treatment. Accordingly, these results indicate the therapeutic potential of CoQ10 supplementation in the treatment of OP poisoning. However, higher doses may be required to engender therapeutic efficacy.
- Subjects :
- 0301 basic medicine
RA1190
medicine.medical_specialty
Insecticides
Cell Survival
Ubiquinone
Methyl Parathion
Mitochondrion
Biochemistry
03 medical and health sciences
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Methyl-parathion
Electron Transport Complex III
0302 clinical medicine
Organophosphate
Internal medicine
Cell Line, Tumor
Dichlorvos
medicine
Citrate synthase
Humans
QD
Viability assay
Diminution
Coenzyme Q10
Neurons
Original Paper
biology
business.industry
Electron Transport Complex II
General Medicine
Mitochondria
030104 developmental biology
Endocrinology
chemistry
Chloropyrifos
Toxicity
biology.protein
Chlorpyrifos
business
030217 neurology & neurosurgery
RC
Subjects
Details
- Language :
- English
- ISSN :
- 15736903 and 03643190
- Volume :
- 46
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Neurochemical Research
- Accession number :
- edsair.doi.dedup.....0c1388a355227d01f42bd4ccda277cc1