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Simkania negevensis may produce long-lasting infections in human pneumocytes and endometrial cells
- Source :
- Pathogens and disease, vol. 75, no. 1, pp. 1, Pathogens and Disease
- Publication Year :
- 2017
-
Abstract
- Simkania negevensis is a novel Chlamydia-related bacterium and the founding member of the Simkaniaceae family within the Chlamydiales order. Little is known about the biology and pathogenesis of this bacterium. So far, S. negevensis has been considered as an amoebal symbiont, but its natural host remains unknown. Moreover, evidence of human exposition has been reported worldwide and an association with pneumonia and bronchiolitis is suspected. Here, we evaluated the ability of S. negevensis to replicate in potential environmental reservoirs, namely amoebae and arthropods, as well as in mammalian cells (Vero cells, pneumocytes and endometrial cells) and further evaluated the characteristics of its replicative vacuole. We demonstrated that S. negevensis efficiently replicates in all cell lines tested, with the shortest doubling time and an increased adhesion observed in pneumocytes. Our work highlights the specificities of the Simkania-containing vacuole compared to other Chlamydiales; contrarily to Chlamydia trachomatis, S. negevensis does not disrupt the Golgi apparatus. Importantly, our work suggests that S. negevensis infection is associated with few cytopathic effects and might persist for a prolonged time in infected cells. Further evaluation of its implication in human diseases is required; an implication in chronic or subacute respiratory infections might be suspected.<br />Simkania negevensis, an emerging Chlamydia-related bacterium, efficiently grows in human cells, suggesting a potential implication in human diseases, and harbors growth characteristics that differ from other known Chlamydiales.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Chlamydiaceae
030106 microbiology
Vacuole
medicine.disease_cause
Microbiology
Simkaniaceae
03 medical and health sciences
Chlorocebus aethiops
medicine
Alveolar Epithelial Cells/microbiology
Amoeba/microbiology
Animals
Cercopithecus aethiops
Chlamydiaceae/classification
Chlamydiaceae/physiology
Chlamydiaceae/ultrastructure
Endometritis/microbiology
Epithelial Cells/microbiology
Female
Humans
Pneumonia/microbiology
Vero Cells
Chlamydia-related bacteria
Chlamydiales order
free-living amoebae
intracellular bacteria
pneumonia
Immunology and Allergy
Amoeba
Chlamydia
General Immunology and Microbiology
biology
Intracellular parasite
Epithelial Cells
General Medicine
Pneumonia
medicine.disease
biology.organism_classification
3. Good health
Infectious Diseases
Chlamydiales
Alveolar Epithelial Cells
Vero cell
Simkania negevensis
Chlamydia trachomatis
Endometritis
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Pathogens and disease, vol. 75, no. 1, pp. 1, Pathogens and Disease
- Accession number :
- edsair.doi.dedup.....0c09ab85401d6494d5370e255bbcdfa2