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Consequences of organ choice in describing bacterial pathogen assemblages in a rodent population
- Source :
- Epidemiology and Infection, Epidemiology and Infection, Cambridge University Press (CUP), 2017, 145 (14), pp.3070-3075. 〈10.1017/S0950268817001893〉, Epidemiology and Infection, Cambridge University Press (CUP), 2017, 145 (14), pp.3070-3075. ⟨10.1017/S0950268817001893⟩, Epidemiol Infect
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- SUMMARYHigh-throughput sequencing technologies now allow for rapid cost-effective surveys of multiple pathogens in many host species including rodents, but it is currently unclear if the organ chosen for screening influences the number and identity of bacteria detected. We used 16S rRNA amplicon sequencing to identify bacterial pathogens in the heart, liver, lungs, kidneys and spleen of 13 water voles (Arvicola terrestris) collected in Franche-Comté, France. We asked if bacterial pathogen assemblages within organs are similar and if all five organs are necessary to detect all of the bacteria present in an individual animal. We identified 24 bacteria representing 17 genera; average bacterial richness for each organ ranged from 1·5 ± 0·4 (mean ± standard error) to 2·5 ± 0·4 bacteria/organ and did not differ significantly between organs. The average bacterial richness when organ assemblages were pooled within animals was 4·7 ± 0·6 bacteria/animal; Operational Taxonomic Unit accumulation analysis indicates that all five organs are required to obtain this. Organ type influences bacterial assemblage composition in a systematic way (PERMANOVA, 999 permutations, pseudo-F4,51 = 1·37, P = 0·001). Our results demonstrate that the number of organs sampled influences the ability to detect bacterial pathogens, which can inform sampling decisions in public health and wildlife ecology.
- Subjects :
- 0301 basic medicine
Operational taxonomic unit
MESH: Sequence Analysis, DNA
Epidemiology
MESH : Prevalence
MESH : Tissue Distribution
Rodent Diseases
RNA, Ribosomal, 16S
bacterial pathogens
Prevalence
MESH : DNA, Bacterial
Tissue Distribution
MESH: Animals
Pathogen
[SDV.EE]Life Sciences [q-bio]/Ecology, environment
education.field_of_study
biology
Ecology
Arvicolinae
Microbiota
MESH : Rodent Diseases
3. Good health
MESH: RNA, Ribosomal, 16S
Infectious Diseases
France
rodent-borne pathogen
DNA, Bacterial
030106 microbiology
Population
Short Report
Zoology
[ SDV.EE ] Life Sciences [q-bio]/Ecology, environment
03 medical and health sciences
Animals
MESH: Microbiota
MESH : Bacteria
MESH: Tissue Distribution
Arvicola terrestris
education
MESH : France
MESH: Prevalence
[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology
Bacteria
Host (biology)
tissue selection
Bacteria Present
Sequence Analysis, DNA
MESH: Arvicolinae
biology.organism_classification
MESH: DNA, Bacterial
MESH: France
MESH : Arvicolinae
MESH : RNA, Ribosomal, 16S
MESH: Bacteria
High-Throughput Sequencing
030104 developmental biology
MESH : Microbiota
Species richness
MESH: Rodent Diseases
MESH : Animals
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
MESH : Sequence Analysis, DNA
Subjects
Details
- Language :
- English
- ISSN :
- 09502688 and 14694409
- Database :
- OpenAIRE
- Journal :
- Epidemiology and Infection, Epidemiology and Infection, Cambridge University Press (CUP), 2017, 145 (14), pp.3070-3075. 〈10.1017/S0950268817001893〉, Epidemiology and Infection, Cambridge University Press (CUP), 2017, 145 (14), pp.3070-3075. ⟨10.1017/S0950268817001893⟩, Epidemiol Infect
- Accession number :
- edsair.doi.dedup.....0c02a437e110bf6e612acdb01d8d9e23