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- Source :
- Antioxidants, Volume 10, Issue 6, Antioxidants, Vol 10, Iss 961, p 961 (2021)
- Publication Year :
- 2021
-
Abstract
- Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss of endothelial function and integrity leads to cardiovascular diseases and multiorgan inflammation. The protective effects of the H2S-donor erucin, an isothiocyanate purified by Eruca sativa Mill. seeds, were evaluated on human endothelial and vascular smooth muscle cells. In particular, erucin actions were evaluated on cell viability, ROS, caspase 3/7, inflammatory markers levels and the endothelial hyperpermeability in an inflammatory model associated with high glucose concentrations (25 mM, HG). Erucin significantly prevented the HG-induced decrease in cell viability as well as the increase in ROS, caspase 3/7 activation, and TNF-α and IL-6 levels. Similarly, erucin suppressed COX-2 and NF-κB upregulation associated with HG exposure. Erucin also caused a significant inhibition of p22phox subunit expression in endothelial cells. In addition, erucin significantly prevented the HG-induced increase in endothelial permeability as also confirmed by the quantification of the specific markers VE-Cadherin and ZO-1. In conclusion, our results assess anti-inflammatory and antioxidant effects by erucin in vascular cells undergoing HG-induced inflammation and this protection parallels the preservation of endothelial barrier properties.
- Subjects :
- 0301 basic medicine
Vascular smooth muscle
Antioxidant
Physiology
vascular wall
medicine.medical_treatment
Eruca sativa Mill
Clinical Biochemistry
hydrogen sulfide
Pharmacology
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Isothiocyanate
vascular inflammation
Erucin
Vascular inflammation
H2S-donor
biology
Hydrogen sulfide
030220 oncology & carcinogenesis
endothelial permeability
medicine.symptom
isothiocyanate
erucin
Vascular wall
Inflammation
Caspase 3
RM1-950
Article
03 medical and health sciences
Downregulation and upregulation
Endothelial permeability
medicine
Viability assay
Molecular Biology
Cell Biology
030104 developmental biology
chemistry
S-donor
biology.protein
H
2
Therapeutics. Pharmacology
P22phox
Subjects
Details
- ISSN :
- 20763921
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Antioxidants (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....0c008c2df8a6ccd087aaa8fc749ab451