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Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways
- Source :
- Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020), Pichler, M J, Yamada, C, Shuoker, B, Alvarez-Silva, C, Gotoh, A, Leth, M L, Schoof, E, Katoh, T, Sakanaka, M, Katayama, T, Jin, C, Karlsson, N G, Arumugam, M, Fushinobu, S & Abou Hachem, M 2020, ' Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways ', Nature Communications, vol. 11, no. 1, 3285 . https://doi.org/10.1038/s41467-020-17075-x, Pichler, M J, Yamada, C, Shuoker, B, Alvarez-Silva, C, Gotoh, A, Leth, M L, Schoof, E, Katoh, T, Sakanaka, M, Katayama, T, Jin, C, Karlsson, N G, Arumugam, M, Fushinobu, S & Abou Hachem, M 2020, ' Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways ', Nature Communications, vol. 11, 3285 . https://doi.org/10.1038/s41467-020-17075-x, Nature Communications
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- The early life human gut microbiota exerts life-long health effects on the host, but the mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of Clostridiales from the Roseburia-Eubacterium group, associated with protection from colorectal cancer, immune- and metabolic disorders is enigmatic. Here, we describe catabolic pathways that support the growth of Roseburia and Eubacterium members on distinct human milk oligosaccharides (HMOs). The HMO pathways, which include enzymes with a previously unknown structural fold and specificity, were upregulated together with additional glycan-utilization loci during growth on selected HMOs and in co-cultures with Akkermansia muciniphila on mucin, suggesting an additional role in enabling cross-feeding and access to mucin O-glycans. Analyses of 4599 Roseburia genomes underscored the preponderance and diversity of the HMO utilization loci within the genus. The catabolism of HMOs by butyrate-producing Clostridiales may contribute to the competitiveness of this group during the weaning-triggered maturation of the microbiota.<br />The assembly and maturation of the early life microbiome has life-long effects on human health. Here, the authors combine omics, functional assays and structural analyses to characterize the catabolic pathways that support the growth of butyrate producing Clostridiales members from the Roseburia and Eubacterium, on distinct human milk oligosaccharides.
- Subjects :
- DYNAMICS
0301 basic medicine
Oligosaccharides
General Physics and Astronomy
Biochemistry
BREAST-MILK
Eubacterium
lcsh:Science
health care economics and organizations
Clostridiales
Multidisciplinary
biology
Butyrates
GEN. NOV
HEALTH
Roseburia
BLOOD-GROUP-A
Akkermansia muciniphila
Colon
Science
HUMAN GUT MICROBIOME
030106 microbiology
BIFIDOBACTERIUM
Weaning
Butyrate
Microbiology
PROTEIN-SEQUENCE
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Verrucomicrobia
SDG 3 - Good Health and Well-being
Polysaccharides
Humans
BACTERIAL DIVERSITY
PURIFICATION
Milk, Human
Catabolism
Mucin
Infant, Newborn
Mucins
Infant
Akkermansia
General Chemistry
biology.organism_classification
Gastrointestinal Microbiome
Metabolism
030104 developmental biology
bacteria
lcsh:Q
Bifidobacterium
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....0bfe4ae8224ba4969779cf6ca9735cab