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Effects of treadmill exercise on anxiety-like behavior in association with changes in estrogen receptors ERα, ERβ and oxytocin of C57BL/6J female mice
- Source :
- IBRO Neuroscience Reports, Vol 11, Iss, Pp 164-174 (2021), IBRO Neuroscience Reports
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Exercise can reduce the incidence of stress-related mental diseases, such as depression and anxiety. Control group was neither exposed to CVMS nor TRE (noCVMS/noTRE). Females were tested and levels of serum17-beta-oestradiol (E2), estrogen receptors α immunoreactive neurons (ERα-IRs), estrogen receptors β immunoreactive neurons (ERβ-IRs) and oxytocin immunoreactive neurons (OT-IRs) were measured. The results showed there’s increased anxiety-like behaviors for mice from CVMS/noTRE, CVMS/higher speed TRE (CVMS/HTRE) and noCVMS/HTRE groups when they were put in open field and elevated maze tests. They had lower serum E2 levels than mice from CVMS/low-moderate speed TRE (CVMS/LMTRE), noCVMS/LMTRE and noCVMS/noTRE groups. The three groups of CVMS/noTRE, CVMS/HTRE and noCVMS/HTRE mice had more ERα-IRs and less ERβ-IRs in the medial preoptic area (mPOA), bed nucleus of the stria terminalis (BNST) and medial amygdala (MeA), hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SON). The number of OT-IRs in PVN and SON of CVMS/noTRE, CVMS/HTRE and noCVMS/HTRE mice was also lower than that of mice from CVMS/LMTRE, noCVMS/LMTRE and noCVMS/noTRE groups. Interestingly, CVMS/LMTRE and noCVMS/LMTRE mice were similar to noCVMS/noTRE mice in that they did not show anxiety, while CVMS/HTRE and noCVMS/HTRE mice did not, which were similar to the mice in CVMS/noTRE. We propose that LMTRE instead of HTRE changes the serum concentration of E2. ERβ/ERα ratio and OT level in the brain may be responsible for the decrease in anxiety-like behavior in female mice exposed to anxiety-inducing stress conditions.<br />Highlights • CVMS/LMTRE did not show anxiety. • noCVMS/LMTRE did not show anxiety. • ERβ/ERα ratio decreas anxiety. • OT decreas anxiety.
- Subjects :
- medicine.medical_specialty
OF, open field test
TRE, treadmill exercise
E2, 17-beta-oestradiol
Estrogen receptor
Treadmill exercise
Neurosciences. Biological psychiatry. Neuropsychiatry
Amygdala
Supraoptic nucleus
Open field
HRP, horseradishperoxidase
HTRE, higher speed TRE
Chronic variable moderate stress (CVMS)
mPOA, medial preopticarea
Estrogen receptor β (ERβ)
Internal medicine
Estrogen receptor α (ERα)
Treadmill exercise (TRE)
ERβ-IRs, estrogen receptor β immunoreactive neurons
Medicine
BNST, bed nucleus of the stria terminalis
LMTRE, low-moderate speed TRE
business.industry
CVMS, chronic variable moderate stress
General Neuroscience
MeA, medial amygdaloid nucleus
ELISA, enzyme-linked immunosorbent assay
PBS, phosphatebufferedsolution
EPM, elevated plusmazetest
HPA, hypothalamic–pituitary–adrenal
SON, supraoptic nucleus
Stria terminalis
medicine.anatomical_structure
Endocrinology
Oxytocin
OT-IRs, Oxytocin immunoreactive neurons
ERα-IRs, estrogen receptors αimmunoreactive neurons
Oxytocin (OT)
business
Nucleus
hormones, hormone substitutes, and hormone antagonists
Research Paper
PVN, paraventricular nucleus
medicine.drug
RC321-571
Subjects
Details
- Language :
- English
- ISSN :
- 26672421
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- IBRO Neuroscience Reports
- Accession number :
- edsair.doi.dedup.....0bf20ff38b0b81369a96926d8a6b833f