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Deuterium nuclear magnetic resonance investigation of bacteriophage M13 coat protein in dimyristoylphosphatidylcholine liposomes using palmitic acid as a probe
- Source :
- Biochimica et biophysica acta-protein structure and molecular enzymology, 944, 507-515, Biochimica et biophysica acta-protein structure and molecular enzymology 944 (1988)
- Publication Year :
- 1988
-
Abstract
- The effect of incorporation of various amounts of M13 bacteriophage coat protein on the bilayer order and acyl chain motion in dimyristoylphosphatidylcholine (DMPC) liposomes has been investigated using deuterium NMR of specifically deuterated palmitic acid as a bilayer probe, phosphorus NMR and additional spin-label electron spin resonance (ESR). The secondary structure of the M13 coat protein in these bilayers was determined from circular dichroism spectra. Phosphorus NMR spectra of the mixed liposomes are characteristic for DMPC organized in bilayers, also after incorporation of various levels of M13 protein. Circular dichroism spectra of the coat protein indicate that the protein conformation is predominantly a β-structure (more than 75%). Various incorporation levels of M13 coat protein do not affect the order of the deuterium-labelled positions along the acyl chain at the carbon-2, 9 and 16 positions. In contrast, the spin-spin relaxation times decrease at higher protein levels, especially at the carbon-16 position. The spin-label ESR spectra of the same system using 14-doxylstearic acid as a label show a second, motionally restricted component, that is not observed by deuterium NMR. The NMR and ESR results are consistent with a model in which the fatty acid molecules are in a fast two-site exchange (at a rate of approx. 10 7 Hz) between the sites in the bulk of the lipid bilayer and the motionally restricted sites on the coat protein.
- Subjects :
- Deuterium NMR
Magnetic Resonance Spectroscopy
Protein Conformation
Biophysics
Phospholipid
Palmitic Acid
2H
Palmitic Acids
Biochemistry
Coliphages
chemistry.chemical_compound
Membrane Lipids
Nuclear magnetic resonance
Protein structure
Capsid
Phosphorus-31 NMR spectroscopy
Lipid bilayer
Protein secondary structure
Coat protein
M13 bacteriophage
biology
Bilayer
Circular Dichroism
Cell Membrane
Cell Biology
(Bacteriophage)
NMR, 2H
biology.organism_classification
NMR
Liposome
Biofysica
chemistry
Solubility
Liposomes
Dimyristoylphosphatidylcholine
Subjects
Details
- ISSN :
- 00063002 and 01674838
- Volume :
- 944
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....0bd3ed570f1fe67dafc824a3e5df994b