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Regulation of fibronectin EDA exon alternative splicing: possible role of RNA secondary structure for enhancer display
- Source :
- Europe PubMed Central, Scopus-Elsevier
- Publication Year :
- 1999
-
Abstract
- The fibronectin primary transcript undergoes alternative splicing in three noncoordinated sites: the cassette-type EDA and EDB exons and the more complex IIICS region. We have shown previously that an 81-nucleotide region within the EDA exon is necessary for exon recognition and that this region contains at least two splicing-regulatory elements: a polypurinic enhancer (exonic splicing enhancer [ESE]) and a nearby silencer element (exonic splicing silencer [ESS]). Here, we have analyzed the function of both elements in different cell types. We have mapped the ESS to the nucleotide level, showing that a single base change is sufficient to abolish its function. Testing of the ESE and ESS elements in heterologous exons, individually or as part of the complete EDA regulatory region, showed that only the ESE element is active in different contexts. Functional studies coupled to secondary-structure enzymatic analysis of the EDA exon sequence variants suggest that the role of the ESS element may be exclusively to ensure the proper RNA conformation and raise the possibility that the display of the ESE element in a loop position may represent a significant feature of the exon splicing-regulatory region.
- Subjects :
- Molecular Sequence Data
Exonic splicing enhancer
Gene Expression
Computational biology
Biology
Regulatory Sequences, Nucleic Acid
Primary transcript
Nucleic acid secondary structure
Exon
RNA Precursors
Tumor Cells, Cultured
Animals
Humans
Point Mutation
RNA, Messenger
Enhancer
Molecular Biology
Exonic splicing silencer
Genetics
Base Sequence
Alternative splicing
Cell Biology
Exons
Fibronectins
Alternative Splicing
Regulatory sequence
Nucleic Acid Conformation
Subjects
Details
- ISSN :
- 02707306
- Volume :
- 19
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular biology
- Accession number :
- edsair.doi.dedup.....0baa0fbf44f8063074d226217a1a6e7d