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Overexpression of PeMIPS1 confers tolerance to salt and copper stresses by scavenging reactive oxygen species in transgenic poplar
- Source :
- Tree physiology. 38(10)
- Publication Year :
- 2017
-
Abstract
- Myo-inositol is a vital compound in plants. As the key rate-limiting enzyme in myo-inositol biosynthesis, l-myo-inositol-1-phosphate synthase (MIPS) is regarded as a determinant of the myo-inositol content in plants. The up-regulation of MIPS genes can increase the myo-inositol content, thereby enhancing the plant's resistance to a variety of stresses. However, there are few reports on the roles of myo-inositol and the identification of MIPS in woody trees. In this study, a MIPS gene, named as PeMIPS1, was characterized from Populus euphratica Oliv. The heterologous expression of PeMIPS1 compensated for inositol production in the yeast inositol auxotrophic mutant ino1 and the phenotypic lesions of the atmips1-2 mutant, an Arabidopsis MIPS1 knock-out mutant. A subcellular location analysis showed that the PeMIPS1-GFP fusion was localized in the nucleus and cytoplasm, but not in the chloroplasts, indicating that PeMIPS1 represented the cytosolic form of MIPS in P. euphratica. Interestingly, PeMIPS1 was not only inducible by drought and high salinity, but also by CuSO4 treatment. The transgenic poplar lines overexpressing PeMIPS1 had greater plant heights, shoot biomasses and survival rates than the wild type during the salt- or copper-stress treatment, and this was accompanied by an increase in the myo-inositol content. The overexpression of PeMIPS1 resulted in the increased activities of antioxidant enzymes and the accumulation of ascorbate, a key nonenzymatic antioxidant in plant, which partly accounted for the enhanced reactive oxygen species-scavenging capacity and the lowered hydrogen peroxide and malondialdehyde levels in the transgenic poplar. To the best of our knowledge, this study is the first to report the roles of MIPS genes in the tolerance to copper stress.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Mutant
Arabidopsis
Plant Science
Genetically modified crops
Saccharomyces cerevisiae
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
Stress, Physiological
Soil Pollutants
Inositol
chemistry.chemical_classification
Reactive oxygen species
biology
fungi
Wild type
food and beverages
Plant physiology
Salt Tolerance
biology.organism_classification
Plants, Genetically Modified
030104 developmental biology
Populus
chemistry
Biochemistry
Myo-Inositol-1-Phosphate Synthase
Heterologous expression
Reactive Oxygen Species
Populus euphratica
Copper
010606 plant biology & botany
Subjects
Details
- ISSN :
- 17584469
- Volume :
- 38
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Tree physiology
- Accession number :
- edsair.doi.dedup.....0b9f023969d62aa0b488d7d7965a13ed