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Dehydration-induced alterations in chloroplast proteome and reprogramming of cellular metabolism in developing chickpea delineate interrelated adaptive responses
Dehydration-induced alterations in chloroplast proteome and reprogramming of cellular metabolism in developing chickpea delineate interrelated adaptive responses
- Source :
- Plant Physiology and Biochemistry. 146:337-348
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Chloroplast, the energy organelle unique to photosynthetic eukaryotes, executes several crucial functions including photosynthesis. While chloroplast development and function are controlled by the nucleus, environmental stress modulated alterations perceived by the chloroplasts are communicated to the nucleus via retrograde signaling. Notably, coordination of chloroplast and nuclear gene expression is synchronized by anterograde and retrograde signaling. The chloroplast proteome holds significance for stress responses and adaptation. We unraveled dehydration-induced alterations in the chloroplast proteome of a grain legume, chickpea and identified an array of dehydration-responsive proteins (DRPs) primarily involved in photosynthesis, carbohydrate metabolism and stress response. Notably, 12 DRPs were encoded by chloroplast genome, while the rest were nuclear-encoded. We observed a coordinated expression of different multi-subunit protein complexes viz., RuBisCo, photosystem II and cytochrome b6f, encoded by both chloroplast and nuclear genome. Comparison with previously reported stress-responsive chloroplast proteomes showed unique and overlapping components. Transcript abundance of several previously reported markers of retrograde signaling revealed relay of dehydration-elicited signaling events between chloroplasts and nucleus. Additionally, dehydration-triggered metabolic adjustments demonstrated alterations in carbohydrate and amino acid metabolism. This study offers a panoramic catalogue of dehydration-responsive signatures of chloroplast proteome and associated retrograde signaling events, and cellular metabolic reprograming.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chloroplasts
Nuclear gene
Proteome
Physiology
Plant Science
01 natural sciences
Genome
03 medical and health sciences
Organelle
Genetics
Humans
Plant Proteins
Dehydration
biology
Cytochrome b6f complex
RuBisCO
food and beverages
Cicer
Cell biology
Chloroplast
030104 developmental biology
biology.protein
Retrograde signaling
010606 plant biology & botany
Subjects
Details
- ISSN :
- 09819428
- Volume :
- 146
- Database :
- OpenAIRE
- Journal :
- Plant Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....0b9a3c39fc45f6b373e78d7696668d60
- Full Text :
- https://doi.org/10.1016/j.plaphy.2019.11.034