Back to Search
Start Over
In vitro antitumor potential of astaxanthin nanoemulsion against cancer cells via mitochondrial mediated apoptosis
- Source :
- International Journal of Pharmaceutics. 560:334-346
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Astaxanthin and alpha-tocopherol have various biological potential with induction of intracellular ROS production in cytosol, endoplasmic reticulum and mitochondrial site. The present study was performed to prepare nanoemulsion (NEs) formulation of astaxanthin and alpha-tocopherol with sodium caseinate (AS-AT/SC NEs) using spontaneous emulsification and ultrasonication for analyzing intracellular ROS production in apoptosis. NEs was characterized with standard analysis, which revealed a high stability of normal pH with a small size and unique zeta potential of spherical structure droplets with no toxicity and faster cell migration. It induced ROS production and confirmed using fluorescent stains due to their unique physicochemical and functional properties. Therefore, it played a significant role in the induction of oxidative stress inside the cell as dose-dependent cytotoxicity at different concentrations. AS-AT/SC NEs with protective effect in maintaining intracellular ROS, oxidative stress and mitochondrial membrane potential to reduced apoptosis morphology in cancer cells through mitochondria-mediated apoptosis that inhibits cell death, thus cell survival was initiated at greater extent. Thereby exhibiting a significant remarkable therapeutic effect in cancer field. To best of our knowledge, this is first study concluded that induction of apoptosis potential by AS-AT/SC NEs emerged as a potential way to eradicate cancer cells.
- Subjects :
- Programmed cell death
Cell Survival
Chemistry, Pharmaceutical
alpha-Tocopherol
Cell
Pharmaceutical Science
Apoptosis
02 engineering and technology
Xanthophylls
medicine.disease_cause
030226 pharmacology & pharmacy
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Line, Tumor
Neoplasms
medicine
Humans
Particle Size
Cytotoxicity
Membrane Potential, Mitochondrial
Dose-Response Relationship, Drug
Chemistry
Caseins
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Mitochondria
Cell biology
Oxidative Stress
Cytosol
medicine.anatomical_structure
Cancer cell
Nanoparticles
Emulsions
Reactive Oxygen Species
0210 nano-technology
Oxidative stress
Intracellular
Subjects
Details
- ISSN :
- 03785173
- Volume :
- 560
- Database :
- OpenAIRE
- Journal :
- International Journal of Pharmaceutics
- Accession number :
- edsair.doi.dedup.....0b95800ddb2a44458a7368fd7e810981