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Production of manganese peroxidase and laccase in a solid-state bioreactor and modeling of enzyme production kinetics
- Source :
- Bioprocess and biosystems engineering. 38(1)
- Publication Year :
- 2014
-
Abstract
- Lignin-modifying enzymes have various promising applications such as biobleaching, biopulping, the functionalization of lignocellulosic materials, the modification of wood fibers, the remediation of contaminated soil and effluents, as well as improvement of the enzymatic hydrolysis of lignocellulosic substrates. In this study, the production of laccase and manganese peroxidase (MnP) in solid-state cultivation was examined. Oat husks were used as an inexpensive substrate for the white-rot fungus Cerrena unicolor PM170798 (FBCC 387). The addition of a fines fraction (consisting of oat flour and finely ground husks) enhanced MnP production fivefold and laccase production almost threefold. The enzyme production was studied first on a 100 g scale, and the cultivation experiments were then repeated at a larger laboratory-scale (4 kg) in a solid-state bioreactor. High enzyme activity levels were obtained (MnP: 340 nkat g(-1) DM, laccase: 470 nkat g(-1) DM). In addition, the correlation between the CO2 evolution rate and enzyme production was mathematically modeled from the bioreactor experimental data. The model parameters could be used to predict enzyme production.
- Subjects :
- Laccase
biology
Avena
Chemistry
Substrate (chemistry)
Bioengineering
General Medicine
Models, Theoretical
biology.organism_classification
Pulp and paper industry
Enzyme assay
Polyporaceae
Kinetics
Bioreactors
Biochemistry
Peroxidases
Manganese peroxidase
Enzymatic hydrolysis
Bioreactor
biology.protein
Cerrena unicolor
Industrial and production engineering
Biotechnology
Subjects
Details
- ISSN :
- 16157605
- Volume :
- 38
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Bioprocess and biosystems engineering
- Accession number :
- edsair.doi.dedup.....0b8da7ca5321a3a28fde2bb18448b341