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Newcastle disease virus infection triggers HMGB1 release to promote the inflammatory response
- Source :
- Virology. 525
- Publication Year :
- 2018
-
Abstract
- High mobility group box 1 (HMGB1) is a key member of the "danger associated molecular patterns" (DAMPs) which plays important roles in systemic inflammation and has a pathogenic role in infectious diseases like viral or bacterial infections. Newcastle disease virus (NDV) infection is proved to cause intense inflammatory responses that result in excessive cellular apoptosis and tissue damage, but the function of HMGB1 in NDV-induced cytokine storm has not been elucidated. Here, we report that HMGB1 is a significant inflammation factor in NDV infection. HMGB1 is widely distributed in chicken tissues, and the secretion of it was induced by NDV infection both in DF-1 and A549 cells. Moreover, inhibiting the secretion of HMGB1, NDV replication was not significantly reduced, but it is involved in NDV-induced NF-κB activation and the inflammatory response. Further investigation showed that HMGB1 promotes inflammatory cytokine production through the RAGR, TLR2, and TLR4 receptors. HMGB1-RAGE interaction also takes parts in activation of ERK1/2 and JNK induced by NDV infection. Neutralizing HMGB1 in vivo, chicken viability was increased. Pathological changes and inflammatory cytokines expression were reduced under NDV infection, which further confirmed the pathogenic roles of HMGB1 in inflammatory responses. Thus, our findings show that HMGB1 contributes to the inflammatory cytokine storm induced by NDV infection, which played a critical role in viral pathogenesis.
- Subjects :
- 0301 basic medicine
animal structures
animal diseases
viruses
medicine.medical_treatment
Viral pathogenesis
Newcastle Disease
Newcastle disease virus
chemical and pharmacologic phenomena
Inflammation
HMGB1
Systemic inflammation
Virus Replication
Antibodies
Proinflammatory cytokine
03 medical and health sciences
chemistry.chemical_compound
Virology
medicine
Animals
Humans
HMGB1 Protein
biology
NF-κB
medicine.disease
Specific Pathogen-Free Organisms
030104 developmental biology
Cytokine
chemistry
A549 Cells
embryonic structures
Immunology
biology.protein
medicine.symptom
Cytokine storm
Chickens
Subjects
Details
- ISSN :
- 10960341
- Volume :
- 525
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....0b6ab0458b257a9838f2bcf94e1f32f8