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Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
- Source :
- Journal of synchrotron radiation. 29(Pt 3)
- Publication Year :
- 2021
-
Abstract
- X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.
Details
- ISSN :
- 16005775
- Volume :
- 29
- Issue :
- Pt 3
- Database :
- OpenAIRE
- Journal :
- Journal of synchrotron radiation
- Accession number :
- edsair.doi.dedup.....0b5f88cbefe47ca8832d6d1eec28cc82