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Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline

Authors :
Simone Sala
Yuhe Zhang
Nathaly De La Rosa
Till Dreier
Maik Kahnt
Max Langer
Lars B. Dahlin
Martin Bech
Pablo Villanueva-Perez
Sebastian Kalbfleisch
Source :
Journal of synchrotron radiation. 29(Pt 3)
Publication Year :
2021

Abstract

X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.

Details

ISSN :
16005775
Volume :
29
Issue :
Pt 3
Database :
OpenAIRE
Journal :
Journal of synchrotron radiation
Accession number :
edsair.doi.dedup.....0b5f88cbefe47ca8832d6d1eec28cc82