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A comprehensive analysis of recruitment and transactivation potential of K-Rta and K-bZIP during reactivation of Kaposi's sarcoma-associated herpesvirus
- Source :
- Ellison, Thomas J; Izumiya, Yoshihiro; Izumiya, Chie; Luciw, Paul A; & Kung, Hsing-Jien. (2009). A comprehensive analysis of recruitment and transactivation potential of K-Rta and K-bZIP during reactivation of Kaposi's sarcoma-associated herpesvirus.. Virology, 387(1), 76-788. UC Office of the President: California HIV/AIDS Research Program. Retrieved from: http://www.escholarship.org/uc/item/3783w85s
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma. K-Rta and K-bZIP are two major viral transcription factors that control reactivation of this virus. Here we report a genome-wide analysis of transcriptional capacity by evaluation of a comprehensive library of 83 putative KSHV promoters. In reporter assays, 34 viral promoters were activated by K-Rta, whereas K-bZIP activated 21 promoters. When K-Rta and K-bZIP were combined, 3 K-Rta responsive promoters were repressed by K-bZIP. The occupancy of K-Rta and K-bZIP across KSHV promoters was analyzed by chromatin immunoprecipitation with a viral promoter-chip in BCBL-1 cells. In addition, acetylation of local histones was examined to determine accessibility of promoters during latency and reactivation. Finally, 10 promoters were selected to study the dynamics of transcription factor recruitment. This study provides a comprehensive overview of the responsiveness of KSHV promoters to K-Rta and K-bZIP, and describes key chromatin changes during viral reactivation.
- Subjects :
- viruses
Microarray
medicine.disease_cause
Virus Replication
Transactivation
Medicine and Health Sciences
Promoter Regions, Genetic
0303 health sciences
Kaposi's sarcoma
virus diseases
physiology [Promoter Regions, Genetic]
Chromatin immunoprecipitation
Chromatin
3. Good health
Basic-Leucine Zipper Transcription Factors
Herpesvirus 8, Human
Transcription
Gene Expression Regulation, Viral
Transcriptional Activation
chemistry
metabolism
physiology [Trans-Activators]
KSHV
Biology
Article
Immediate early protein
Immediate-Early Proteins
Cell Line
03 medical and health sciences
Virology
medicine
Humans
Kaposi's sarcoma-associated herpesvirus
Transcription factor
HHV-8
030304 developmental biology
K-Rta/ORF50
030306 microbiology
genetics
physiology [Herpesvirus 8, Human]
K-bZIP/K8
metabolism [Immediate-Early Proteins]
Promoter
biochemical phenomena, metabolism, and nutrition
medicine.disease
DNA, Viral
genetics [DNA, Viral]
Trans-Activators
Virus Activation
Gene expression
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 387
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....0b5c61e0800cfe399a7c05cfc2d596ea
- Full Text :
- https://doi.org/10.1016/j.virol.2009.02.016