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Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
- Source :
- Journal of Pharmacological Sciences, Vol 148, Iss 1, Pp 103-107 (2022)
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- We have reported that nicotine has a neurotrophic action on peripheral adrenergic nerves in vivo, which is mediated by α7 nicotinic acetylcholine receptors (nAChRs). To clarify the possible mechanisms, the present study further investigated the effect of nicotine on neurite outgrowth in tyrosine hydroxylase (TH)-positive superior cervical ganglia (SCG) cells isolated from neonatal rats in vitro. Nicotine at low concentrations (0.01–0.3 mM) increased the number of neurite outgrowths in TH-immunopositive SCG cells, while high concentrations of nicotine (1–10 mM) gradually reduced it, and only 10 mM nicotine was markedly inhibited compared to the control. A 100 μM of nicotine-induced increase in neurite numbers depended on the exposure time and was inhibited by treatment with the nAChR antagonist hexamethonium (Hex) and α7 nAChR antagonist α-bungarotoxin (α-Bgtx). The nicotine (10 mM)-induced a significant decrease in neurite outgrowth in SCG, which was perfectly canceled by Hex to the control level but not by α-Bgtx. These results suggest that nicotine has a regulatory neurotrophic action mediated by both α7 nAChR and other subtypes in TH-positive SCG cells of rats.
- Subjects :
- Nicotine
medicine.medical_specialty
Nicotinic acetylcholine receptors
alpha7 Nicotinic Acetylcholine Receptor
Neurite
Neuronal Outgrowth
RM1-950
Superior Cervical Ganglion
chemistry.chemical_compound
Internal medicine
Neurites
medicine
Rat superior cervical ganglia cells
Animals
Nerve Growth Factors
Cells, Cultured
Pharmacology
Control level
biology
Tyrosine hydroxylase
Neurite outgrowth
Antagonist
Rats
medicine.anatomical_structure
Endocrinology
chemistry
Cervical ganglia
biology.protein
Molecular Medicine
Hexamethonium
Therapeutics. Pharmacology
Neurotrophin
medicine.drug
Subjects
Details
- ISSN :
- 13478613
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmacological Sciences
- Accession number :
- edsair.doi.dedup.....0b59aa02f2e84fee72969691ad75e3bc