Analysis of homing potential of marrow-derived mononuclear cells in an experimentally-induced brain stroke mouse model

Primary objective: To analyse the efficacy of bone marrow-derived mononuclear cells (MNCs) in traversing the blood–brain barrier (BBB) in an experimental model of stroke.Methods and procedures: The middle cerebral artery occlusion (MCAo) mouse model was established and behavioural and histological analysis was performed, subsequently the carboxyfluorescein diacetate (CFDA)-labelled MNCs were transplanted through the tail vein immediately after 23 hours of reperfusion. The fluorescence microscopic analysis of the brain sections was analysed in both acute and sub-acute phases of transplantation.Results: The neurological deficit was confirmed by TTC staining and contra lateral turning behaviour. After 2 and 7 days of transplantation, the CFDA-labelled MNCs were observed in the infarcted regions along the line of cortex.Conclusion: The presence of the CFDA-labelled cells in the ischaemic injured brain lesions proved homing of the implanted MNCs in the infarcted regions of the brain. The successful homing of M...