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Curdlan-Conjugated PLGA Nanoparticles Possess Macrophage Stimulant Activity and Drug Delivery Capabilities
- Source :
- Pharmaceutical Research.
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- There is significant interest in the application of nanoparticles to deliver immunostimulatory signals to cells. We hypothesized that curdlan (immune stimulating polymer) could be conjugated to PLGA and nanoparticles from this copolymer would possess immunostimulatory activity, be non-cytotoxic and function as an effective sustained drug release system.Carbodiimide chemistry was employed to conjugate curdlan to PLGA. The conjugate (C-PLGA) was characterized using (1)H and (13)C NMR, FTIR, DSC and TGA. Nanoparticles were synthesized using an emulsion-solvent evaporation technique. Immunostimulatory activity was characterized in THP-1 derived macrophages. MTT assay and real-time impedance measurements were used to characterize polymer and nanoparticle toxicity and uptake in macrophages. Drug delivery capability was assessed across Caco-2 cells using rifampicin as a model drug.Spectral characterization confirmed successful synthesis of C-PLGA. C-PLGA nanoparticles enhanced phosphorylated ERK production in macrophages indicating cell stimulation. Nanoparticles provided slow release of rifampicin across Caco-2 cells. Polymers but not nanoparticles altered the adhesion profiles of the macrophages. Impedance measurements suggested Ca(2+) dependent uptake of nanoparticles by the macrophages.PLGA nanoparticles with macrophage stimulating and sustained drug delivery capabilities have been prepared. These nanoparticles can be used to stimulate macrophages and concurrently deliver drug in infectious disease therapy.
- Subjects :
- Cell Membrane Permeability
beta-Glucans
Cell Survival
Chemistry, Pharmaceutical
medicine.medical_treatment
Molecular Sequence Data
Antitubercular Agents
Biological Transport, Active
Pharmaceutical Science
Nanoparticle
Nanotechnology
Curdlan
Conjugated system
Excipients
chemistry.chemical_compound
Drug Delivery Systems
Immune system
Polylactic Acid-Polyglycolic Acid Copolymer
medicine
Humans
Macrophage
Pharmacology (medical)
Lactic Acid
Pharmacology
Macrophages
Organic Chemistry
Stimulation, Chemical
Stimulant
PLGA
Carbohydrate Sequence
Intestinal Absorption
chemistry
Drug delivery
Biophysics
Nanoparticles
Molecular Medicine
Caco-2 Cells
Rifampin
Polyglycolic Acid
Biotechnology
Subjects
Details
- ISSN :
- 1573904X and 07248741
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical Research
- Accession number :
- edsair.doi.dedup.....0b3f81e31e60d01a7e0baa8133e0d745