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Normal B Cell Homeostasis Requires B Cell Activation Factor Production by Radiation-resistant Cells
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 2003
- Publisher :
- Rockefeller University Press, 2003.
-
Abstract
- The cellular source of B cell activation factor (BAFF) required for peripheral B cell survival/maturation is unknown. To determine the nature of BAFF-producing cells we established and analyzed reciprocal bone marrow (BM) chimeras with wild-type (WT) and BAFF-deficient mice. The results revealed that BAFF production by radiation-resistant stromal cells is completely sufficient to provide a necessary signal for B cell survival/maturation, as BAFF−/− BM cells transferred into lethally irradiated WT mice gave rise to normal numbers of follicular (FO) and marginal zone (MZ) B cell subpopulations. On the other hand, transfer of WT BM into BAFF−/− lethally irradiated mice resulted only in minimal reconstitution of mature FO B cells and no restoration of MZ B cells. Thus, in the absence of BAFF+/+ stromal cells, BAFF production by BM-derived cells, presumably by macrophages, dendritic cells, and/or neutrophils, was not at all sufficient to support normal B cell homeostasis. Interestingly, immunization of both types of chimeras stimulated high levels of antigen-specific antibody secretion, indicating that either stromal cell– or hematopoietic cell–derived BAFF is sufficient for B cell antibody responses.
- Subjects :
- bone marrow
Stromal cell
Cell Survival
Immunology
Naive B cell
Bone Marrow Cells
Cell Separation
Article
Mice
stomatognathic system
B cell homeostasis
B-Cell Activating Factor
homeostasis
medicine
Animals
Immunology and Allergy
B-cell activating factor
B cell
Mice, Knockout
B-Lymphocytes
B cells
Radiation
biology
Tumor Necrosis Factor-alpha
Membrane Proteins
Flow Cytometry
Molecular biology
Mice, Inbred C57BL
stomatognathic diseases
Haematopoiesis
medicine.anatomical_structure
Radiation Chimera
biology.protein
BAFF
Bone marrow
Stromal Cells
Antibody
Spleen
Subjects
Details
- ISSN :
- 15409538 and 00221007
- Volume :
- 198
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....0b3f7c9d639369124f6dd8a8cef74a53
- Full Text :
- https://doi.org/10.1084/jem.20030789