Additional file 1 of Interfollicular epidermal stem-like cells for the recreation of the hair follicle epithelial compartment

Additional file 1: Fig. S1. Phenotype of the CD49fbri/CD71dim subpopulation. A) Representative image of fluorescent activated cell sorting (FACS) dotplot of the whole population of epidermal keratinocytes and the gatting of the subpopulations of cells, based on the expression of the CD49f and CD71 markers, accompanied by the respective cellular percentages. CD49fbri/CD71dim cells represent epidermal stem-like cells, CD49fbri/CD71bri subpopulation are transit-amplifying (TA) cells, while differentiated (Diff.) cells are characterized by a CD49fdim phenotype. Epidermal stem-like cells derived colonies growing in the inactivated feeders (B) 4 and (C) 6 days after seeding. Data shown are mean ± SEM. Scale bars are 50 μm. Fig. S2. Human cell detection within skin explants from the hair reconstitution assay. Staining with a human-specific probe demonstrates that, 6 weeks after cells injection, there were no human cells remaining in the wound area in the controls (A,B) and in the condition where interfollicular epidermal stem-like keratinocytes (EpSlKCs) and DP cells were co-grafted (C). Likewise, no staining was observed in the mice tissue (D), whereas nuclear orange-pink staining was observed in human positive control specimens (E,F), demonstrating the specificity of the staining. Scale bars are 200 μm, for (A-D) and 50 μm for (E,F). Table S1. List of antibodies used for flow cytometry studies. Table S2. List of antibodies used for immunofluorescence studies.