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Duodenal fatty acid sensor and transporter expression following acute fat exposure in healthy lean humans
- Source :
- Clinical Nutrition. 36:564-569
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Summary Background & aims Free fatty acids (FFAs) and their derivatives are detected by G-protein coupled receptors (GPRs) on enteroendocrine cells, with specific transporters on enterocytes. It is unknown whether acute fat exposure affects FFA sensors/transporters, and whether this relates to hormone secretion and habitual fat intake. Methods We studied 20 healthy participants (10M, 10F; BMI: 22 ± 1 kg/m 2 ; age: 28 ± 2 years), after an overnight fast, on 2 separate days. On the first day, duodenal biopsies were collected endoscopically before, and after, a 30-min intraduodenal (ID) infusion of 10% Intralipid ® , and relative transcript expression of FFA receptor 1 (FFAR1), FFA receptor 4 (FFAR4), GPR119 and the FFA transporter, cluster of differentiation-36 (CD36) was quantified from biopsies. On the second day, ID Intralipid ® was infused for 120-min, and plasma concentrations of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) evaluated. Habitual dietary intake was assessed using food frequency questionnaires (FFQs). Results ID Intralipid ® increased expression of GPR119, but not FFAR1, FFAR4 and CD36, and stimulated CCK and GLP-1 secretion. Habitual polyunsaturated fatty acid (PUFA) consumption was negatively associated with basal GPR119 expression. Conclusions GPR119 is an early transcriptional responder to duodenal lipid in lean humans, although this response appeared reduced in individuals with high PUFA intake. These observations may have implications for downstream regulation of gut hormone secretion and appetite. This study was registered as a clinical trial with the Australia and New Zealand Clinical Trial Registry (Trial number: ACTRN12612000376842).
- Subjects :
- Blood Glucose
Male
0301 basic medicine
Non-Randomized Controlled Trials as Topic
CD36
Appetite
Critical Care and Intensive Care Medicine
Body Mass Index
Receptors, G-Protein-Coupled
0302 clinical medicine
Glucagon-Like Peptide 1
Surveys and Questionnaires
Insulin
Medicine
Phospholipids
media_common
Cholecystokinin
chemistry.chemical_classification
Nutrition and Dietetics
biology
Fatty Acids
Glucagon-like peptide-1
Fatty Acids, Unsaturated
Free fatty acid receptor
Emulsions
Female
Polyunsaturated fatty acid
Adult
medicine.medical_specialty
Duodenum
Enteroendocrine Cells
media_common.quotation_subject
030209 endocrinology & metabolism
Diet, High-Fat
Gastrointestinal Hormones
03 medical and health sciences
Thinness
Internal medicine
Free fatty acid receptor 1
Humans
business.industry
Fatty acid
Diet
Soybean Oil
030104 developmental biology
Endocrinology
chemistry
biology.protein
business
Subjects
Details
- ISSN :
- 02615614
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Clinical Nutrition
- Accession number :
- edsair.doi.dedup.....0b2cf2529d3467921a756bbbadc6059d