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Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons
- Source :
- Angewandte Chemie. 128:5017-5021
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Newly synthesized proteins constitute an important subset of the proteome involved in every cellular process, yet existing chemical tools used to study them have major shortcomings. Herein we report a suite of cell-permeable puromycin analogues capable of being metabolically incorporated into newly synthesized proteins in different mammalian cells, including neuronal cells. Subsequent labeling with suitable bioorthogonal reporters, in both fixed and live cells, enabled direct imaging and enrichment of these proteins. By taking advantage of the mutually orthogonal reactivity of these analogues, we showed multiplexed labeling of different protein populations, as well as quantitative measurements of protein dynamics by fluorescence correlation spectroscopy, could be achieved in live-cell environments.
- Subjects :
- Neurons
0301 basic medicine
Cellular process
Protein dynamics
Direct imaging
Fluorescence correlation spectroscopy
General Chemistry
General Medicine
010402 general chemistry
01 natural sciences
Catalysis
0104 chemical sciences
chemistry.chemical_compound
03 medical and health sciences
030104 developmental biology
Biochemistry
chemistry
Puromycin
Protein Biosynthesis
Proteome
Protein biosynthesis
Humans
Bioorthogonal chemistry
HeLa Cells
Subjects
Details
- ISSN :
- 00448249
- Volume :
- 128
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie
- Accession number :
- edsair.doi.dedup.....0b297eac3eb571b5517949c8d8965738