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Evaluation of leucocyte esterase reagent strip test for the rapid bedside diagnosis of spontaneous bacterial peritonitis
- Source :
- Indian Journal of Gastroenterology. 29:74-77
- Publication Year :
- 2010
- Publisher :
- Springer Science and Business Media LLC, 2010.
-
Abstract
- Spontaneous bacterial peritonitis (SBP) is a common serious complication of cirrhosis, and carries a high morbidity and mortality. Rapid diagnosis and prompt treatment of this condition may improve survival of such patients.To validate the diagnostic efficacy of a leukocyte esterase reagent (LER) strip test for rapid, bedside diagnosis of SBP.We prospectively studied 175 patients with liver cirrhosis and ascites [mean age 48 (SD 16.4) years; 146 men] between August 2007 and December 2008. Alcohol was the most common (124 of 175; 70.8%) cause of liver cirrhosis. All patients underwent abdominal paracentesis, and the ascitic fluid was processed for cell count, LER strip (Magistik 10) test and culture. Two different cut-offs for calling the LER strip test positive were tried, namely when the color turned light blue [grade 2:125 polymorphonuclear leucocytes (PMNL)/microL] or it turned purple (grade 3:500 PMNL/microL). Sensitivity, specificity, positive predictive value, negative predictive value were calculated, using PMN count by microscopy exceeding 250 PMNL/microL.LER strip using the more stringent purple-color cut off to diagnose SBP had a sensitivity of 92% and specificity of 100%. The corresponding figures using the light-blue color cut-off were 97% and 89%, respectively.LER strip testing of ascitic fluid is a rapid, cheap and sensitive bed side tool for the diagnosis of SBP.
- Subjects :
- Adult
Liver Cirrhosis
Male
medicine.medical_specialty
Cirrhosis
Point-of-Care Systems
Peritonitis
Gastroenterology
Cohort Studies
Spontaneous bacterial peritonitis
Predictive Value of Tests
Internal medicine
medicine
Ascitic Fluid
Humans
Reagent Strips
Reagent strip
business.industry
Peritoneal fluid
Leucocyte esterase
Bacterial Infections
Middle Aged
Hepatology
medicine.disease
Surgery
ROC Curve
Predictive value of tests
Female
Complication
business
Carboxylic Ester Hydrolases
Subjects
Details
- ISSN :
- 09750711 and 02548860
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Indian Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....0b152a6dda3d79637740262eccc17b6a