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Eosinophils from eosinophilic oesophagitis patients have T cell suppressive capacity and express FOXP3
- Source :
- Clinical and Experimental Immunology. 187:455-465
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Summary Eosinophilic esophagitis (EoE) is an antigen-driven T cell-mediated chronic inflammatory disease where food and environmental antigens are thought to have a role. Human eosinophils express the immunoregulatory protein galectin-10 and have T cell suppressive capacity similar to regulatory T cells (Tregs). We hypothesized that one function of eosinophils in EoE might be to regulate the T cell-driven inflammation in the oesophagus. This was tested by evaluating the suppressive capacity of eosinophils isolated from the blood of adult EoE patients in a mixed lymphocyte reaction. In addition, eosinophilic expression of forkhead box protein 3 (FOXP3), the canonical transcription factor of Tregs, was determined by conventional and imaging flow cytometry, quantitative polymerase chain reaction (qPCR), confocal microscopy and immunoblotting. It was found that blood eosinophils from EoE patients had T cell suppressive capacity, and that a fraction of the eosinophils expressed FOXP3. A comparison of EoE eosinophils with healthy control eosinophils indicated that the patients' eosinophils had inferior suppressive capacity. Furthermore, a higher percentage of the EoE eosinophils expressed FOXP3 protein compared with the healthy eosinophils, and they also had higher FOXP3 protein and mRNA levels. FOXP3 was found in the cytosol and nucleus of the eosinophils from both the patients and healthy individuals, contrasting with the strict nuclear localization of FOXP3 in Tregs. To conclude, these findings suggest that the immunoregulatory function of eosinophils may be impaired in EoE.
- Subjects :
- Adult
Male
0301 basic medicine
Adolescent
T cell
Immunology
Inflammation
Biology
T-Lymphocytes, Regulatory
Leukocyte Count
Young Adult
03 medical and health sciences
0302 clinical medicine
Antigen
Eosinophilic
medicine
Humans
Immunology and Allergy
Eosinophilic esophagitis
Aged
FOXP3
Forkhead Transcription Factors
Original Articles
Eosinophilic Esophagitis
Middle Aged
respiratory system
medicine.disease
Mixed lymphocyte reaction
Eosinophils
030104 developmental biology
Real-time polymerase chain reaction
medicine.anatomical_structure
Female
medicine.symptom
030215 immunology
Subjects
Details
- ISSN :
- 13652249 and 00099104
- Volume :
- 187
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Immunology
- Accession number :
- edsair.doi.dedup.....0af9407cf2b4595d91170f15c344b038
- Full Text :
- https://doi.org/10.1111/cei.12898